Millipore Sigma Vibrant Logo

MABF457 Anti-CD86 (B7-2) Antibody (mouse), FITC, clone GL-1

This rat monoclonal antibody Anti-CD86 (B7-2) (mouse), FITC, clone GL-1 validated for use in IHC, Immunofluorescence & Flow Cytometry for the detection of CD86 (B7-2).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Cd86 UniProt Number: P42082
View Products on Sigmaaldrich.com
MABF457
100 µg  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      Special Offers

       

      Contact Customer Service

      Overview

      Replacement Information

      Special Offers

      100% Performance Guaranteed

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIHC, IF, FCRFITCMonoclonal Antibody
      Description
      Catalogue NumberMABF457
      DescriptionAnti-CD86 (B7-2) Antibody (mouse), FITC, clone GL-1
      Alternate Names
      • T-lymphocyte activation antigen CD86
      • Activation B7-2 antigen
      • Early T-cell costimulatory molecule 1
      • ETC-1
      • CD86
      Background InformationThe GL-1 antibody reacts with mouse CD86, also known as B7-2, an 80 kDa cell surface protein which is a ligand for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 can also bind a second B7 ligand known as CD80 (B7-1). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. The GL-1 antibody may be used as a marker for CD86 expression on B cells, macrophages, and dendritic cells.
      References
      Product Information
      FormatFITC
      PresentationPurified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rat monoclonal antibody Anti-CD86 (B7-2) (mouse), FITC, clone GL-1 validated for use in IHC, Immunofluorescence & Flow Cytometry for the detection of CD86 (B7-2).
      Key Applications
      • Immunohistochemistry
      • Immunofluorescence
      • Flow Cytometry
      Biological Information
      ImmunogenImmunogen information for this antibody is proprietary information.
      CloneGL-1 (GL1)
      Concentration0.5 mg/mL
      HostRat
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Cd86
      Purification MethodSize Exclusion Chromatography
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

      Flow Cytometry Analysis: 0.125 µg of this antibody detected CD86 (B7-2) in one million C57Bl/6 splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF457 04053252960468

      Related Products & Applications

      Related Products

      Catalogue Number Description  
      MABF724 Anti-CD86 (B7-2) Antibody (mouse), FITC, clone GL-1 Show Pricing & Availability
      MABF726 Anti-CD86 (B7-2) Antibody (mouse), PE, clone GL-1 Show Pricing & Availability
      MABF458 Anti-CD86 (B7-2) Antibody (mouse), PE, clone GL-1 Show Pricing & Availability
      MABF723 Anti-CD86 (B7-2) Antibody (mouse), APC, clone GL-1 Show Pricing & Availability

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies