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MABT326 Anti-CEACAM3/5 Antibody, clone 308/3-3

Anti-CEACAM Antibody, clone 308/3-3 is an antibody against CEACAM3/5 for use in Western Blotting, ELISA, Flow Cytometry, Immunoprecipitation.

Anti-CEACAM3/5 Antibody, clone 308/3-3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: CEACAM3, CD66D, CGM1, CEACAM5, CEA UniProt Number: P40198
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MABT326
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISA, FC, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT326
      DescriptionAnti-CEACAM3/5 Antibody, clone 308/3-3
      Alternate Names
      • Carcinoembryonic antigen-related cell adhesion molecule 3
      • Carcinoembryonic antigen CGM1
      • CD66d
      • Carcinoembryonic antigen-related cell adhesion molecule 5
      • Carcinoembryonic antigen
      • CEA
      • Meconium antigen 100
      • CD66e
      Background InformationCarcinoembryonic antigen-related cell adhesion molecule 3 (UniProt P40198; also known as Carcinoembryonic antigen CGM1, CD66d) is encoded by the CEACAM3 (also known as CD66D, CGM1) gene (Gene ID 1084) in human. The carcinoembryonic antigen- (CEA-) related cell adhesion molecules (CEACAMs) constitute a 12-member subgroup of the CEA family of immunoglobulin-related proteins first described in 1965 (PMID 4953873). CEACAMs are reported to participate in diverse physiological processes, including cell adhesion, differentiation, proliferation, and survival, as well as in carcinogenesis and bacterial pathogenesis. CEACAM molecules from neighbouring cells can interact via their respective extracellular N-terminal IgV-like domain and mediate cell-to-cell adhesion through trans-oligomerization. CEACAM molecules within the same cell can also undergo transmembrane domain-mediated cis-oligomerization, an event important for sustaining downstream cellular signaling. Athough CEACAM1 is shown to utilize its cytoplasmic domain for transducing cellular signaling, not all CEACAM members are expressed with a significant cytoplasmic domain, and others (CEACAM5/6/7/8) are GPI-anchored without even a transmembrane domain. Human CEACAM3 and CEACAM4 are two CEACAMs whose extracellular region is composed of only one V-type Ig-like extracellular domain (a.a. 35-142 of CEACAM3). Human CEACAM5 (UniProt P06731), on the other hand, is a GPI-anchored CEACAM with the most extracellular Ig domains, 7 in all.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CEACAM Antibody, clone 308/3-3 is an antibody against CEACAM3/5 for use in Western Blotting, ELISA, Flow Cytometry, Immunoprecipitation.
      Key Applications
      • Western Blotting
      • ELISA
      • Flow Cytometry
      • Immunoprecipitation
      Application NotesELISA Analysis: 10 μg/mL from a representative lot detected CHO cell-expressed full-length glycosylated human CEACAM3 and CEACAM5, but not CEACAM1 (CEACAM1-4L), CEACAM4, CEACAM6, CEACAM7, or CEACAM8 (Courtesy of Dr. Bernhard B. Singer, University of Duisburg-Essen, Germany).
      Flow Cytometry Analysis: 10 μg/mL from a representative lot immunostained CHO cell transfectants expressing human CEACAM3 and CEACAM5, but not CHO cells expressing human CEACAM1 (CEACAM1-4L), CEACAM4, CEACAM6, CEACAM7, or CEACAM8 (Courtesy of Dr. Bernhard B. Singer, University of Duisburg-Essen, Germany).
      Immunoprecipitation Analysis: 5 μg from a representative lot immunoprecipitated exogenously expressed full-length glycosylated human CEACAM5 from 500 μg of lysate (in 600 μL) from transfected HeLa cells (Courtesy of Dr. Bernhard B. Singer, University of Duisburg-Essen, Germany).
      Western Blotting Analysis: 10 μg/mL from a representative lot detected exogenously expressed full-length glycosylated human CEACAM5 in 50 μg of lysate from transfected HeLa cells (Courtesy of Dr. Bernhard B. Singer, University of Duisburg-Essen, Germany).
      Biological Information
      ImmunogenHuman IgG1 Fc-tagged recombinant human CEACAM3 extracellular domain.
      EpitopeExtracellular domain.
      Clone308/3-3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 308/3-3 reacts with human CEACAM3 and CEACAM5, but not human CEACAM1/4/6/7/8, rat CEACAM1, or mouse CEACAM1 & CEACAM2.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteNot Mouse, Not Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CEACAM3
      • CD66D
      • CGM1
      • CEACAM5
      • CEA
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~30/180 kDa (CEACAM3/CEACAM5) observed. 27.09/19.40/22.75 kDa (human CEACAM3 isoform CGM1A/CGM1B/CGM1C) and 76.80/76.72 kDa (human CEACAM5 isoform 1/2) calculated. Target bands appear smeary and larger than the calculated molecular weights due to glycosylation. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting Analysis in lysates of CEACAM3 & CEACAM5 transfected CHO cells.

      Western Blotting Analysis: 2.0 μg/mL of this antibody detected exogenously expressed full-length glycosylated human CEACAM3 and CEACAM5 in 10 μg of lysates from transfected CHO cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT326 04055977349597