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MABC1147 Anti-CIP2A Antibody, clone 6G8-G1

Detect CIP2A using this mouse monoclonal Anti-CIP2A Antibody, clone 6G8-G1, Cat. No. MABC1147, tested in Western Blotting.

Anti-CIP2A Antibody, clone 6G8-G1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: KIAA1524:CIP2A UniProt Number: Q8TCG1
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MABC1147
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1147
      DescriptionAnti-CIP2A Antibody, clone 6G8-G1
      Alternate Names
      • Protein CIP2A
      • Cancerous inhibitor of PP2A
      • p90 autoantigen
      Background InformationProtein CIP2A (UniProt: Q8TCG1; also known as CIP2A, Cancerous inhibitor of PP2A, p90 autoantigen) is encoded by the KIAA1524 (also known as CIP2A) gene (Gene ID: 57650) in human. CIP2A is a single pass membrane protein that exhibits dynamic changes in localization, including the cytoplasm and centrosome, depending on the stage of the cell cycle. It is expressed at low levels in most tissues, however, it is overexpressed in several cancers, including head-and-neck squamous cell carcinomas and liver cancer cells. Levels of CIP2A are correlated with the proliferation of human cancer cells and with cancer progression. It acts as an oncoprotein and inhibits protein phosphatase 2A activity and thereby stabilizes c-Myc in human malignancies. It promotes anchorage-independent cell growth and tumor formation. Higher activity of c-Myc rapidly induces the activation of cyclin E-cdk2 kinase activity. Concurrently, the release of p27Kip1 from Cdk2 complexes enhances cell proliferation. In CIP2A depleted cells, centrosome separation and the mitotic spindle dynamics were impaired, which leads to the activation of spindle assembly checkpoint signaling and, ultimately, delayed cell division time. CIP2A is also reported to interact with NIMA (never in mitosis gene A)-related kinase 2 (NEK2) during G2/M phase and enhances NEK2 kinase activity that facilitates centrosome separation in a PP1- and PP2A-independent manner. (Ref.: Jeong, AL et al. (2014). J. Biol. Chem. 289(1): 28-40).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect CIP2A using this mouse monoclonal Anti-CIP2A Antibody, clone 6G8-G1, Cat. No. MABC1147, tested in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenHis-tagged recombinant fragment from the N-terminal region of human CIP2A.
      EpitopeN-terminus
      Clone6G8-G1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6G8-G1 specifically detects CIP2A in HEK293 cells overexpressing CIP2A.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KIAA1524:CIP2A
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~102 kDa observed; 102.18 calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cells overexpressing CIP2A.

      Western Blotting Analysis: 1 µg/mL of this antibody detected CIP2A in 10 µg of lysate from HEK293 cells overexpressing CIP2A.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1147 04054839169540