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ABS2088 Anti-CLIC4

Detect Chloride intracellular channel protein 4 using this rabbit polyclonal Anti-CLIC4, Cat. No. ABS2088, used in Western Blotting and Immunofluorescence, Western Blotting, Immunohistochemistry (Paraffin) and Immunoprecipitation.

Anti-CLIC4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: CLIC4 UniProt Number: Q9XSA7
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ABS2088
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RWB, IF, IH(P), IPRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS2088
      DescriptionAnti-CLIC4
      Alternate Names
      • Intracellular chloride ion channel protein p64H1
      Background InformationChloride intracellular channel protein 4 (UniProt: Q9XSA7; also known as Intracellular chloride ion channel protein p64H1, CLIC4) is encoded by the CLIC4 gene (Gene ID: 286823) in bovine. CLIC4, a member of the CLIC family of proteins. CLIC family members can change from a globular, soluble state to a state where the N-terminal domain is inserted into the membrane and functions as chloride channel. A conformation change of the N-terminal domain is thought to expose hydrophobic surfaces that trigger membrane insertion. CLIC4 is ubiquitously expressed and is present in both soluble and membrane-associated forms. It is present in the cytosol as a diffusible protein, but is reported in endoplasmic reticulum, trans-Golgi network, mitochondria, caveolae, dense-core secretory vesicles in the central nervous system, and in nuclei of differentiating keratinocytes. CLIC4 is rapidly recruited to the plasma membrane by lysophosphatidic acid (LPA) and serum, indicating its possible role in exocytic endocytic trafficking. CLIC4 also participates in such diverse processes as angiogenesis, differentiation, migration and wound healing. It is reported to stimulate both the internalization and serum/ LPA-induced recycling of beta1 integrin. In developing and developed kidneys, CLIC4 is enriched in the proximal tubule epithelial cells where it is considered to be essential for luminal delivery, microvillus morphogenesis, and endo-lysosomal biogenesis. CLIC4-null mice embryos exhibit impaired renal tubulogenesis and have smaller kidneys with fewer glomeruli and less dense peritubular capillary network. (Ref.: Edwards, JC et al. (2014). BMC Nephrology 15:54; Argenzio, A et al. (2014). J. Cell Sci.127 (24): 5189-5203).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect Chloride intracellular channel protein 4 using this rabbit polyclonal Anti-CLIC4, Cat. No. ABS2088, used in Western Blotting and Immunofluorescence, Western Blotting, Immunohistochemistry (Paraffin) and Immunoprecipitation.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot detected CLIC4 in Madin-Darby canine kidney (MDCK) cells (Chou, S.Y., et. al. (2016). Nat Commun. 7:10412).

      Immunohistochemistry Analysis: A representative lot detected CLIC4 in Immunohistochemistry applications (Chou, S.Y., et. al. (2016). Nat Commun. 7:10412).

      Western Blotting Analysis: A representative lot detected CLIC4 in immunoblots of renal lysates harvested from WT and CLIC4-KO postnatal 0 (PN0 mice) (Chou, S.Y., et. al. (2016). Nat Commun. 7:10412).

      Immunofluorescence Analysis: A representative lot detected CLIC4 in postnatal 0 (PN0) mouse renal paraffin and frozen sections (Chou, S.Y., et. al. (2016). Nat Commun. 7:10412).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 158 amino acids from the internal region of bovine Chloride intracellular channel protein 4 (CLIC4).
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects intracellular channel protein 4 in rat brain.
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Bovine.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CLIC4
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~29 kDa observed; 28.73 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue lysate.

      Western Blotting Analysis: 1:500 dilution of this antibody detected CLIC4 in 10 µg of rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2088 04054839168734