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MABE1831-25UG Anti-CSB (ERCC6) Antibody, clone ICSB-3H8

Anti-CSB (ERCC6), clone ICSB-3H8, Cat. No. MABE1831, is a highly specific mouse monoclonal antibody that targets DNA excision repair protein ERCC-6 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Anti-CSB (ERCC6) Antibody, clone ICSB-3H8 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: ERCC6, CSB UniProt Number: Q03468
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MABE1831-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HChIP, FC, ICC, IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1831-25UG
      DescriptionAnti-CSB (ERCC6) Antibody, clone ICSB-3H8
      Alternate Names
      • DNA excision repair protein ERCC-6
      • ATP-dependent helicase ERCC6
      • Cockayne syndrome protein CSB
      Background InformationDNA excision repair protein ERCC-6 (UniProt: Q03468; also known as ATP-dependent helicase ERCC6, Cockayne syndrome protein CSB) is encoded by the ERCC6 (also known as CSB) gene (Gene ID: 2074) in human. CSB is a homodimeric nuclear protein that belongs to the SwItch/ Sucrose nonfermentable family of DNA-dependent ATPases and contains seven characteristic helicase motifs. It is a part of a complex that contains RNA pol I, TFIIH, and XPG, all of which are active in transcription and/or DNA repair. Hence, it is an essential factor involved in transcription-coupled nucleotide excision repair, which allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA. It is shown to be essential for transcription-coupled repair complex formation. It recruits the CSA complex (DCX(ERCC8) complex), nucleotide excision repair proteins and EP300 to the at sites of RNA polymerase II-blocking lesions. Mutations in CSB (ERCC6) gene are known to cause age related macular degeneration and Cockayne syndrome B that is characterized by delayed neural development and severe progressive neurologic degeneration and mental retardation.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-CSB (ERCC6), clone ICSB-3H8, Cat. No. MABE1831, is a highly specific mouse monoclonal antibody that targets DNA excision repair protein ERCC-6 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Immunocytochemistry, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Chromatin Immunoprecipitation (ChIP)
      • Flow Cytometry
      • Immunocytochemistry
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A representative lot detected CSB (ERCC6) in Immunocytochemistry applications (Bradsher, J., et. al. (2002). Mol Cell. 10(4):819-29).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated CSB (ERCC6) in Immunoprecipitation applications (Bradsher, J., et. al. (2002). Mol Cell. 10(4):819-29).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected CSB (ERCC6) in Chromatin Immunoprecipitation applications (Kristensen, U., et. al. (2013). Proc Natl Acad Sci USA. 110(25):E2261-70; Velez-Cruz, R., et. al. (2012). Proc Natl Acad Sci USA. 110(3):E212-20).

      Western Blotting Analysis: A representative lot detected CSB (ERCC6) in Western Blotting applications (Bradsher, J., et. al. (2002). Mol Cell. 10(4):819-29).
      Biological Information
      ImmunogenOvalbumin-conjugated linear Peptide corresponding to 22 amino acids from the C-terminal region of human DNA excision repair protein ERCC-6.
      Clone1CSB-3H8
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1CSB-3H8 specifically detects DNA excision repair protein ERCC-6 in human cells. It targets an epitope with in 22 amino acids from the C-terminal region.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ERCC6
      • CSB
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight168.42 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry in A431 cells.

      Flow Cytometry Analysis: 1 µg of this antibody detected CSB (ERCC6) in one million A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1831-25UG 04054839557903