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MABC1705-100UG Anti-CXCL1 Antibody, clone 2A10

Anti-CXCL1, clone 2A10, Cat. No. MABC1705, is a mouse monoclonal antibody that detects CXCL1 and is tested for use in Flow Cytometry and Western Blotting.

MABC1705-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABC1705-100UG
      DescriptionAnti-CXCL1 Antibody, clone 2A10
      Alternate Names
      • Growth-regulated alpha protein
      • C-X-C motif chemokine 1
      • GRO-alpha(1-73)
      • Melanoma growth stimulatory activity
      • MGSA
      • Neutrophil-activating protein 3
      • NAP-3)
      Background InformationGrowth-regulated alpha protein (UniProt: P09341; also known as C-X-C motif chemokine 1, GRO-alpha(1-73), Melanoma growth stimulatory activity, MGSA, Neutrophil-activating protein 3, NAP-3) is encoded by the CXCL1 (also known as GRO, GRO1, GROA, MGSA, SCYB1) gene (Gene ID: 2919) in human. CXCL1 is a secreted protein of the CXC chemokine family with chemotactic activity for neutrophils. It is synthesized with a signal peptide (aa 1-34), which is subsequently cleaved off to produce the mature protein. Its levels are reported to be very low under basal conditions but increase significantly following active infection. It plays a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. It plays a role in recruiting and activating neutrophils for microbial killing at the tissue site and can also activate the release of proteases and reactive oxygen species. CXCL1 signals through binding to CXCR2, a G-protein coupled receptor. Aberrant expression of CXCL1 has been reported in several cancers and has been associated with oncogenesis, metastasis, angiogenesis and chemoresistance. (Ref.: Sawant, KV., et al. (2016). Sci. Rep. 6; 33123; Yang C., et al. (2019). Int. J. Oncol. 55(3); 684-696).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in PBS with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-CXCL1, clone 2A10, Cat. No. MABC1705, is a mouse monoclonal antibody that detects CXCL1 and is tested for use in Flow Cytometry and Western Blotting.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 1 µg of this antibody detected CXCL1 in one million human PBMC cells.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenFull-length mature recombinant human C-X-C motif chemokine 1 (CXCL1).
      EpitopeUnknown
      Clone2A10
      Concentration1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 2A10 is a mouse monoclonal antibody that detects human C-X-C motif chemokine 1 (CXCL1).
      IsotypeIgG2bκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CXCL1
      • GRO
      • GRO1
      • MSGA
      • SCYB1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~11 kDa observed; 11.30 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting with recombinant human CXCL1.

      Western Blotting Analysis: 1:50,000 dilution of this antibody detected recombinant human CXCL1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1705-100UG 04065265085847