208903 Anti-Calpastatin, Domain IV Mouse mAb (1F7E3D10)

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208903
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~150, ~125, ~90, and ~70 kDa forms of calpastatin that are believed to correspond to proteins translated from different start sites of the calpastatin gene.
      Catalogue Number208903
      Brand Family Calbiochem®
      References
      ReferencesCong, J., et al. 1993. J. Biol. Chem. 268, 25740.
      Lane, R.D., et al. 1992. Exper. Cell Res. 203, 5.
      Product Information
      FormLiquid
      FormulationAscites diluted in PBS.
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesImmunoblotting (1:5000, colorimetric)
      Immunofluorescence (1:200)
      Application CommentsRecognizes multiple forms of calpastatin that are suggested to be a result of translation initiation from different start sites. Epitope is in domain IV (543-673) of human calpastatin. Reacts with calpastatin from several mammalian sources. Does not cross-react with calpain or calmodulin. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogenpurified calpastatin from bovine skeletal muscle
      ImmunogenBovine
      Clone1F7E3D10
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Calpastatin, Domain IV Mouse mAb (1F7E3D10) Certificates of Analysis

      TitleLot Number
      208903

      References

      Reference overview
      Cong, J., et al. 1993. J. Biol. Chem. 268, 25740.
      Lane, R.D., et al. 1992. Exper. Cell Res. 203, 5.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-July-2007 RFH
      ApplicationImmunoblotting (1:5000, colorimetric)
      Immunofluorescence (1:200)
      DescriptionMouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3x3Ag8.653 mouse myeloma cells. Supplied as diluted ascites. Recognizes the ~150, ~125, ~90, and ~70 kDa forms of calpastatin.
      BackgroundCalpastatin is a specific endogenous inhibitor of calpain that has an internal repeat of four homologous domains which allow it to inhibit multiple calpain molecules simultaneously. It functions by binding to calpain when calpain binds Ca2+. When calpastatin is subjected to proteolytic digestion, fragments of calpastatin as small as 15 kDa still retain inhibitory activity.
      HostMouse
      Immunogen speciesBovine
      Immunogenpurified calpastatin from bovine skeletal muscle
      Clone1F7E3D10
      IsotypeIgG₁
      Speciesbovine, human, rat
      FormLiquid
      FormulationAscites diluted in PBS.
      Preservative≤0.1% sodium azide
      CommentsRecognizes multiple forms of calpastatin that are suggested to be a result of translation initiation from different start sites. Epitope is in domain IV (543-673) of human calpastatin. Reacts with calpastatin from several mammalian sources. Does not cross-react with calpain or calmodulin. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesCong, J., et al. 1993. J. Biol. Chem. 268, 25740.
      Lane, R.D., et al. 1992. Exper. Cell Res. 203, 5.