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ABS1671 Anti-Calreticulin Antibody, arginylated (Nt-Glu18)

Detect arginylated mature calreticulin using this rabbit polyclonal Anti-Calreticulin, arginylated (Nt-Glu18), Cat. No. ABS1671, validated for use in ELISA, Immunocytochemistry, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: CALR, CRTC UniProt Number: P27797
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ABS1671
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, ICC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1671
      DescriptionAnti-Calreticulin Antibody, arginylated (Nt-Glu18)
      Alternate Names
      • Calreticulin, Nt-Glu18 arginylated
      • Calregulin, Nt-Glu18 arginylated
      • CRP55, Nt-Glu18 arginylated
      • CRT, Nt-Glu18 arginylated
      • Endoplasmic reticulum resident protein 60, Nt-Glu18 arginylated
      • ERp60, Nt-Glu18 arginylated
      • grp60, Nt-Glu18 arginylated
      • HACBP, Nt-Glu18 arginylated
      Background InformationCalreticulin (UniProt P27797; also known as Calregulin, CRP55, CRT, Endoplasmic reticulum resident protein 60, ERp60, grp60, HACBP) is encoded by the CALR (also known as CRTC) gene (Gene ID 811) in human. Calreticulin (CRT), BiP (GRP78 or heat shock 70 kDa protein 5), and protein disulphide isomerase (PDI) are ER lumen folding factors that, together with other PTM enzymes, assist the process of newly synthesized proteins before their release from ER. CRT, BiP, and PDI themselves are subject to posttranslational signal peptide removal after their synthesis, exposing E18, E19, and D18 at the newly formed N-terminus, respectively. The N-terminus D and E residues of the mature proteins are permissive to arginine-tRNA-protein transferase 1/ATE1-catalyzed arginylation, forming arginylated mature proteins (R-CRT, R-BiP, R-PDI) with altered cellular localization to allow their participation in non-ER functions. Although only the basal levels of R-CRT and R-PDI, but not R-BiP, are generally detectible in non-stimulated cells, upregulated N-terminal arginylation of all three proteins is observed upon cytosolic dsDNA exposure and proteasomal inhibition, indicating a shared role in innate immune responses to invading microbes. In addition, ER stress induction by thapsigargin treatment synergistically boosts proteasomal inhibition-induced upregulation of cellular levels of R-CRT, R-BiP, and R-PDI, suggesting that ER stress accelerates the supply of ER lumenal CRT, BiP, and DPI for N-terminal arginylation.
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      PresentationPurified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect arginylated mature calreticulin using this rabbit polyclonal Anti-Calreticulin, arginylated (Nt-Glu18), Cat. No. ABS1671, validated for use in ELISA, Immunocytochemistry, and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Calreticulin Nt-Glu18 arginylation induction in (18-hr 3 µM MG132 and 200 nM thapsigargin) treated HeLa cells (Courtesy of Yong Tae Kwon, Ph.D. , Seoul National University, Korea).

      Western Blotting Analysis: 0.2 µg/mL from a representative lot detected Calreticulin Nt-Glu18 arginylation induction in (18-hr 3 µM MG132 and 200 nM thapsigargin) treated HeLa cells (Courtesy of Yong Tae Kwon, Ph.D. , Seoul National University, Korea).

      ELISA Analysis: A representative lot detected the immunogen peptide, but not the control peptide without arginylation at the N-terminal Glu18 (Cha-Molstad, H., et al. (2015). Nat. Cell Biol. 17(7):917-929).

      Western Blotting Analysis: A representative lot detected Calreticulin Nt-Glu18 arginylation induction upon arginine-tRNA-protein transferase 1 (ATE1) 1A7A isoform overexpression or transfection of various dsDNAs, including poly(dA:dT), in HeLa cells. Combined proteasome inhibition and ER stress induction by an 18-hr 10 µM MG132 and 100 nM thapsigargin treatment synergized the two drugs' efficacy toward cellular Calreticulin Nt-Glu18 arginylation induction (Cha-Molstad, H., et al. (2015). Nat. Cell Biol. 17(7):917-929).
      Biological Information
      ImmunogenLinear peptide corresponding to the N-terminal sequence of arginylated mature human Calreticulin.
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detected the immunogen peptide, but not the control peptide without arginylation at the N-terminal Glu18 (Cha-Molstad, H., et al. (2015). Nat. Cell Biol. 17(7):917-929).
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CALR
      • CRTC
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~55 kDa observed. 46.6 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Calreticulin Nt-Glu18 arginylation induction in 7.5 µg of lysate from (17-hr 3 µM MG132 and 200 nM thapsigargin) treated HEK293 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1671 04054839083600