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PC41 Anti-Cathepsin B (Ab-3) Rabbit pAb

Anti-Cathepsin B (Ab-3), rabbit polyclonal, recognizes the ~43 kDa latent and ~31 kDa active forms of cathepsin B in breast carcinoma. It is validated for Western blotting and for paraffin sections.

Anti-Cathepsin B (Ab-3) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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PC41
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Pricing & Availability

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PC41-100UG
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      Description
      OverviewRecognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B in breast carcinoma tissues.
      Catalogue NumberPC41
      Brand Family Calbiochem®
      References
      ReferencesBarrett, A.J. and Kirschke, H. 1981. Methods Enzymol. 80, 535.
      Chan, S.J., et al. Proc. Natl. Acad. Sci. USA 83, 7721.
      Barrett, A.J. 1987. Trends Biochem. Sci. 12, 193.
      Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 137.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlSK-HEP-1 concentrated conditioned medium or breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml)
      Paraffin Sections (2.5 µg/ml, trypsin pre-treatment required)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified human liver cathepsin B
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC41-100UG 04055977224597

      Documentation

      Anti-Cathepsin B (Ab-3) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cathepsin B (Ab-3) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC41

      References

      Reference overview
      Barrett, A.J. and Kirschke, H. 1981. Methods Enzymol. 80, 535.
      Chan, S.J., et al. Proc. Natl. Acad. Sci. USA 83, 7721.
      Barrett, A.J. 1987. Trends Biochem. Sci. 12, 193.
      Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 137.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision10-October-2007 RFH
      ApplicationImmunoblotting (1 µg/ml)
      Paraffin Sections (2.5 µg/ml, trypsin pre-treatment required)
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~43 kDa latent and the ~31 kDa active forms of cathepsin B.
      BackgroundCathepsin B (CB) is a normal lysosomal proteinase which is expressed in all cells. It is a cysteine endoproteinase which is structurally and functionally related to the papain family of proteinases. CB is synthesized as an inactive ~43 kDa pro-enzyme which, by removal of a 62 amino acid propeptide, is activated to the single-chain form of ~31 kDa or the two-chain form (~25 and ~5 kDa subunits). Mature CB is normally localized in the lysosomes where it functions in protein turnover. It has also been shown to be capable of degrading extracellular matrix proteins at acidic and neutral pH. Endogenous inhibitors of cysteine proteinases, the cystatins and stefins, may play a major role in regulating their activity. In tumor cells and in cells exposed to mitogens, CB displays altered cellular trafficking resulting in the secretion of the ~43 kDa precursor. Elevated levels of CB correlate with malignancy suggesting that this enzyme may be a useful prognostic marker for several types of human cancers.
      HostRabbit
      Immunogen speciesHuman
      Immunogenpurified human liver cathepsin B
      IsotypeIgG
      Specieshuman
      Positive controlSK-HEP-1 concentrated conditioned medium or breast carcinoma tissue
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesBarrett, A.J. and Kirschke, H. 1981. Methods Enzymol. 80, 535.
      Chan, S.J., et al. Proc. Natl. Acad. Sci. USA 83, 7721.
      Barrett, A.J. 1987. Trends Biochem. Sci. 12, 193.
      Sloane, B.F. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 137.