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IM03 Anti-Cathepsin D (Ab-1) Mouse mAb (BC011)

Anti-Cathepsin D (Ab-1), mouse monoclonal, clone BC011, recognizes latent and mature form and the ~34 kDa subunit in breast carcinoma tissue extracts. It is validated for WB and for paraffin sections.

Anti-Cathepsin D (Ab-1) Mouse mAb (BC011) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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IM03
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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IM03-100UG
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      Description
      OverviewRecognizes the ~52 kDa latent form, the ~48 kDa mature form, and the ~34 kDa subunit of cathepsin D in breast carcinoma tissue extracts.
      Catalogue NumberIM03
      Brand Family Calbiochem®
      References
      ReferencesRochefort, H. 1992. Eur. J. Cancer. 28A, 1780.
      Rochefort, H. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 153.
      Westly, B. and Rochefort, H. 1980. Cell 20, 352.
      Barrett, A.J. 1977. in Proteinases in Mammalian Cells and Tissues, ed. Barret, A.J. (North Holland, New York), pp. 209.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Positive controlSK-HEP-1 concentrated conditioned medium or breast carcinoma tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesImmunoblotting (1 µg/ml)
      Paraffin Sections (5 µg/ml, trypsin pre-treatment required)
      Application CommentsDoes not detect the ~14 kDa subunit of cathespin D, nor will it detect cathespins B or L. Cathepsins are secreted and accumulate in conditioned tissue culture media and can be detected by immunoblotting from this source. Some cell extracts do not contain sufficient cathepsin for detection by immunoblotting. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenmature, human cathepsin D
      ImmunogenHuman
      CloneBC011
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      IM03-100UG 04055977228137

      Documentation

      Anti-Cathepsin D (Ab-1) Mouse mAb (BC011) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cathepsin D (Ab-1) Mouse mAb (BC011) Certificates of Analysis

      TitleLot Number
      IM03

      References

      Reference overview
      Rochefort, H. 1992. Eur. J. Cancer. 28A, 1780.
      Rochefort, H. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 153.
      Westly, B. and Rochefort, H. 1980. Cell 20, 352.
      Barrett, A.J. 1977. in Proteinases in Mammalian Cells and Tissues, ed. Barret, A.J. (North Holland, New York), pp. 209.

      Brochure

      Title
      Cathepsins and Related Products Technical Bulletin
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      ApplicationImmunoblotting (1 µg/ml)
      Paraffin Sections (5 µg/ml, trypsin pre-treatment required)
      DescriptionPurified mouse monoclonal antibody generated by immunizing CB6F1 mice with the specified immunogen and fusing splenocytes with SP2/0 cells. Recognizes the ~52 kDa pro-cathespin D, the ~48 kDa mature cathepsin D, and the ~34 kDa subunit of cathespin D.
      BackgroundCathepsin D is a normal lysosomal proteinase which is expressed in all cells. It is an aspartyl proteinase with a pH optimum in the range of 3-5 and contains two N-linked oligosaccharides. Cathepsin D is synthesized as an inactive 52 kDa pro-enzyme. Activation involves the proteolytic removal of the 43 amino acid pro-fragment and internal cleavage to generate the two chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal which targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, cathepsin D is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence suggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer.
      HostMouse
      Immunogen speciesHuman
      Immunogenmature, human cathepsin D
      CloneBC011
      IsotypeIgG2a
      Specieshuman
      Positive controlSK-HEP-1 concentrated conditioned medium or breast carcinoma tissue
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not detect the ~14 kDa subunit of cathespin D, nor will it detect cathespins B or L. Cathepsins are secreted and accumulate in conditioned tissue culture media and can be detected by immunoblotting from this source. Some cell extracts do not contain sufficient cathepsin for detection by immunoblotting. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesRochefort, H. 1992. Eur. J. Cancer. 28A, 1780.
      Rochefort, H. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1(2), 153.
      Westly, B. and Rochefort, H. 1980. Cell 20, 352.
      Barrett, A.J. 1977. in Proteinases in Mammalian Cells and Tissues, ed. Barret, A.J. (North Holland, New York), pp. 209.