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CC16 Anti-Cdc2/Cdk1 (Ab-3) Mouse mAb (A17.1.1)

Anti-Cdc2/Cdk1 (Ab-3) Mouse mAb (A17.1.1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CC16
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~34 kDa Cdc2/Cdk1 protein in NIH3T3 cells.
      Catalogue NumberCC16
      Brand Family Calbiochem®
      References
      ReferencesDoussis-Anagnostopoulou, I.A., et al. 1994. Histopathol. 24, 335.
      Kobayashi, H., et al. 1992. Mol. Biol. Cell 3, 1279.
      Gould, K., and Nurse, P. 1989. Nature 342, 39.
      Riabowol, K., et al. 1989. Cell 57, 393.
      Draetta, G., and Beach, D. 1987. Cell 54, 17.
      Lee, M.G., and Nurse P. 1987. Nature 327, 31.
      Reed, S.I., et al. 1985. Proc. Natl. Acad. Sci (USA) 82, 4055.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Positive controlAny proliferating cell line
      PreservativeNone
      Applications
      Application ReferencesFrozen Sections Doussis-Anagnostopoulou, I.A., et al. 1994 Histopathol. 24, 335. Immunoprecipitation Kobayashi, H., et al. 1992. Mol. Biol. Cell 3, 1279.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesFlow Cytometry (1 µg/ml)
      Immunoblotting (2.5 µg/ml)
      Frozen Sections (see application references)
      Immunofluorescence (1 µg/ml)
      Immunoprecipitation (see application references)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant Xenopus Cdc2/Cdk1
      ImmunogenXenopus
      Epitopewithin amino acids 220-227 of murine Cdc2/Cdc1
      CloneA17.1.1
      HostMouse
      IsotypeIgG2a
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CC16 0

      Documentation

      Anti-Cdc2/Cdk1 (Ab-3) Mouse mAb (A17.1.1) SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Doussis-Anagnostopoulou, I.A., et al. 1994. Histopathol. 24, 335.
      Kobayashi, H., et al. 1992. Mol. Biol. Cell 3, 1279.
      Gould, K., and Nurse, P. 1989. Nature 342, 39.
      Riabowol, K., et al. 1989. Cell 57, 393.
      Draetta, G., and Beach, D. 1987. Cell 54, 17.
      Lee, M.G., and Nurse P. 1987. Nature 327, 31.
      Reed, S.I., et al. 1985. Proc. Natl. Acad. Sci (USA) 82, 4055.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-August-2007 RFH
      ApplicationFlow Cytometry (1 µg/ml)
      Immunoblotting (2.5 µg/ml)
      Frozen Sections (see application references)
      Immunofluorescence (1 µg/ml)
      Immunoprecipitation (see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with Sp2/0 mouse myeloma cells. Recognizes the ~34 kDa cdc2/cdk1 protein.
      Backgroundcdc2, p34 is the protein product of the human homolog of the yeast cdc2/cdc28 gene. A nuclear protein, cdc2 is associated with the centrosomes during the mitotic process. The cdc2/cdk1 protein, itself a phosphoprotein, has a serine/threonine protein kinase activity and is highly conserved during evolution. Phosphorylation of cdc2/cdk1 occurs at the ATP binding site of the protein. In the phosphorylated form, cdc2/cdk1 fails to bind ATP and the kinase activity of the protein is thereby blocked. cdc2/cdk1 associates with members of the cyclin family of proteins, most notably with cyclins A and B and as such comprise the mitotic cyclin. The kinase activity of cdc2/cdk1 is most readily studied by using histone H1 as a phosphorylation substrate in vitro.
      HostMouse
      Immunogen speciesXenopus
      Immunogenrecombinant Xenopus Cdc2/Cdk1
      Epitopewithin amino acids 220-227 of murine Cdc2/Cdc1
      CloneA17.1.1
      IsotypeIgG2a
      SpeciesXenopus, chicken, guinea pig, human, mouse, rat, woodchuck
      Positive controlAny proliferating cell line
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesDoussis-Anagnostopoulou, I.A., et al. 1994. Histopathol. 24, 335.
      Kobayashi, H., et al. 1992. Mol. Biol. Cell 3, 1279.
      Gould, K., and Nurse, P. 1989. Nature 342, 39.
      Riabowol, K., et al. 1989. Cell 57, 393.
      Draetta, G., and Beach, D. 1987. Cell 54, 17.
      Lee, M.G., and Nurse P. 1987. Nature 327, 31.
      Reed, S.I., et al. 1985. Proc. Natl. Acad. Sci (USA) 82, 4055.
      Application referencesFrozen Sections Doussis-Anagnostopoulou, I.A., et al. 1994 Histopathol. 24, 335. Immunoprecipitation Kobayashi, H., et al. 1992. Mol. Biol. Cell 3, 1279.