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MABF2052-100UL Anti-Chikungunya virus Antibody, clone 3E7b

Anti-Chikungunya virus, clone 3E7b, Cat. No. MABF2052, is a mouse monoclonal antibody that detects E2 protein of Chikungunya virus and has been tested for use in ELISA, Immunocytochemistry, Neutralizing asssay, and Western Blotting.

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MABF2052-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2052-100UL
      DescriptionAnti-Chikungunya virus Antibody, clone 3E7b
      Alternate Names
      • CHIKV
      Background InformationChikungunya virus (CHIKV) that causes distinctive polyarthritis or polyarthralgia with clinical features such as fever, maculopapular rash, and myalgia is transmitted by Aedes spp of mosquitoes. Immune compromised individuals may face serious complications, including encephalitis and mortality. CHIKV genome contains a single-stranded positive-sense RNA that encodes four non-structural proteins known as nsP1, nsP2, nsP3, and nsP4 and also five structural proteins that include a capsid protein, three envelope glycoproteins known as (E1, E2, and E3, and a small molecule known as 6K. The mature alphavirus particles express E1 and E2 heterodimers that form 80 trimeric spikes on the surface of the virion. The ectodomain E1 protein consists of three domains known as D1, DII, and DIII. The DIII domain is an immunoglobulin-like domain connected to D1 and DII by a flexible linker. Both E1 and E2 proteins are responsible for virus entry into host cells. The E2 glycoprotein interacts with a cellular receptor, resulting in the virus internalization and the E1 glycoprotein mediates virus fusion to host cell under low pH conditions. Following the fusion of the viral envelope with the endosomal membrane, the viral genomic RNA is released into the cytoplasm and starts replicating. This monoclonal antibody (3E7b) can bind strongly to native CHIKV surface and potently neutralize virus replication (Ref.: Marsinoul, P., et al. (2014). Virology 464-465; 111-117; Lam, S., et al. (2015). mAbs 7(6), 1178-1194).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in PBS without azide.
      Applications
      ApplicationAnti-Chikungunya virus, clone 3E7b, Cat. No. MABF2052, is a mouse monoclonal antibody that detects E2 protein of Chikungunya virus and has been tested for use in ELISA, Immunocytochemistry, Neutralizing asssay, and Western Blotting.
      Key Applications
      • ELISA
      • Immunocytochemistry
      • Neutralization Assay
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Chikungunya virus in Western Blotting applications (Lam, S., et. al. (2015). MAbs. 7(6):1178-94).

      Immunocytochemistry Analysis: A representative lot detected Chikungunya virus in Immunocytochemistry applications (Lam, S., et. al. (2015). MAbs. 7(6):1178-94).

      Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Chikungunya virus in BHK cells infected with Chikungunya virus. (Courtesy from an independent lab).

      Neutralizing Analysis: A representative lot neutralized Chikungunya virus in Neutralizing applications (Lam, S., et. al. (2015). MAbs. 7(6):1178-94).

      ELISA Analysis: A representative lot detected Chikungunya virus in ELISA applications (Lam, S., et. al. (2015). MAbs. 7(6):1178-94).
      Biological Information
      ImmunogenChikungunya virus
      Clone3E7b
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3E7b detects Chikungunya virus in cells infected with this virus.
      IsotypeIgMκ
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotype Analysis: The identity of this monoclonal antibody is confirmed by isotype test to be mouse IgMk.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2052-100UL 04054839469053

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