Millipore Sigma Vibrant Logo

AP1014 Anti-Cleaved Caspase-9 (Asp³¹⁵) Rabbit pAb

Anti-Cleaved Caspase-9 (Asp³¹⁵) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Cleaved APAF-3 (Asp³¹⁵), Anti-Cleaved CASP9 (Asp³¹⁵), Anti-Cleaved ICE-LAP6 (Asp³¹⁵), Anti-Cleaved Mch6 (Asp³¹⁵)

AP1014
  
Retrieving price...
Price could not be retrieved
Minimum Quantity is a multiple of
Maximum Quantity is
Upon Order Completion More Information
You Saved ()
 
—
Request Pricing
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

       

      Contact Customer Service

      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~35 kDa large active subunit of caspase-9. Does not cross-react with full length caspase-9 or other cleaved caspases.
      Catalogue NumberAP1014
      Brand Family Calbiochem®
      SynonymsAnti-Cleaved APAF-3 (Asp³¹⁵), Anti-Cleaved CASP9 (Asp³¹⁵), Anti-Cleaved ICE-LAP6 (Asp³¹⁵), Anti-Cleaved Mch6 (Asp³¹⁵)
      Application Data
      Detection of human Cleaved Caspase-9 by immunoblotting. Samples: Whole cell extracts from Jurkat cells (lanes 1 and 2) untreated (lane 1) or treated (lane 2) with cytochrome-c and HeLa cell extracts (lanes 3,4,5) untreated (lane 3) or treated with either staurosporine (lane 4) or cytochrome-c (lane 5). Primary antibody: Anti-Cleaved Caspase-9 (Asp315) Rabbit pAb (Cat. No. AP1014) (1:1000). Detection: chemiluminescence.
      References
      ReferencesMitchell, K.O., et al. 2000. Cancer Res. 60, 6318.
      Zou, H., et al. 1999. J. Biol. Chem. 274, 11549.
      Slee, E.A., et al. 1999. J. Cell Biol. 144, 282.
      Product Information
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      Positive controlHeLa cells treated with staurosporine
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000)
      Immunoprecipitation (1:100)
      Application CommentsDoes not cross-react with full length caspase-9 or other cleaved caspases. For immunoblotting, incubate membrane with antibody diluted in 5% nonfat dry milk, 1X TBS, and 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids surrounding the Asp³¹⁵ cleavage site of human caspase-9
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1014 0

      Documentation

      Anti-Cleaved Caspase-9 (Asp³¹⁵) Rabbit pAb Certificates of Analysis

      TitleLot Number
      AP1014

      References

      Reference overview
      Mitchell, K.O., et al. 2000. Cancer Res. 60, 6318.
      Zou, H., et al. 1999. J. Biol. Chem. 274, 11549.
      Slee, E.A., et al. 1999. J. Cell Biol. 144, 282.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      SynonymsAnti-Cleaved APAF-3 (Asp³¹⁵), Anti-Cleaved CASP9 (Asp³¹⁵), Anti-Cleaved ICE-LAP6 (Asp³¹⁵), Anti-Cleaved Mch6 (Asp³¹⁵)
      ApplicationImmunoblotting (1:1000)
      Immunoprecipitation (1:100)
      Application Data
      Detection of human Cleaved Caspase-9 by immunoblotting. Samples: Whole cell extracts from Jurkat cells (lanes 1 and 2) untreated (lane 1) or treated (lane 2) with cytochrome-c and HeLa cell extracts (lanes 3,4,5) untreated (lane 3) or treated with either staurosporine (lane 4) or cytochrome-c (lane 5). Primary antibody: Anti-Cleaved Caspase-9 (Asp315) Rabbit pAb (Cat. No. AP1014) (1:1000). Detection: chemiluminescence.
      DescriptionProtein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~35 kDa large subunit of caspase-9.
      BackgroundCaspase-9 is an important member of the cysteine aspartic acid protease (caspase) family. Upon apoptosis cytochrome c released from mitochondria associates with procaspase-9 (APAF, ~47 kDa) and this complex processes the procaspase-9 into a large active subunit (~17 or ~38 kDa) and a small subunit (~10 kDa) by self-cleavage at Asp315. The cleaved caspase-9 also processes other caspases, including caspases 3 and 7, to start a caspase cascade that leads to programmed cell death. Procaspase-9 can also be cleaved by caspase-3 at Asp330, serving as a positive sign to amplify the apoptotic signal in the caspase activation pathway.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids surrounding the Asp³¹⁵ cleavage site of human caspase-9
      IsotypeIgG
      Specieshuman
      Positive controlHeLa cells treated with staurosporine
      FormLiquid
      FormulationIn 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with full length caspase-9 or other cleaved caspases. For immunoblotting, incubate membrane with antibody diluted in 5% nonfat dry milk, 1X TBS, and 0.1% Tween®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesMitchell, K.O., et al. 2000. Cancer Res. 60, 6318.
      Zou, H., et al. 1999. J. Biol. Chem. 274, 11549.
      Slee, E.A., et al. 1999. J. Cell Biol. 144, 282.