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MABE1152-25UL Anti-Cryptochrome-1 Antibody, clone D46-3361

Anti-Cryptochrome-1, clone D46-3361 Antibody, Cat. No. MABE1152, is a highly specific rat monoclonal antibody, that targets Cryptochrome-1 and has been tested in Western Blotting and Chromatin Immunoprecipitation (ChIP).

Anti-Cryptochrome-1 Antibody, clone D46-3361 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: Cry1 UniProt Number: P97784
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MABE1152-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, ChIPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1152-25UL
      DescriptionAnti-Cryptochrome-1 Antibody, clone D46-3361
      Alternate Names
      • Cry1
      • cryptochrome 1 (photolyase-like)
      • Cryptochrome-1
      • Phll1
      • Cry-1
      Background InformationCryptochrome-1 (UniProt: P97784) is encoded by the Cry1 gene (Gene ID: 12952) in murine species. Cryptochrome-1 is an evolutionarily conserved member of the flavoprotein superfamily that acts as a transcriptional repressor and forms a core component of the circadian clock. It has a photolyase domain at its N-terminal (aa 3-132). Cryptochrome-1 has three FAD biding sites (aa 252, 289, and 355). However, only a minority of the protein molecules contain bound FAD. Transcription and translation of core clock components (CLOCK, NPAS2, ARNTL/BMAL1, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1 and CRY2) play a critical role in rhythm generation, whereas delays imposed by post-translational modifications (PTMs) are important for determining the period (tau) of the rhythms. Disruptions in the circadian rhythms contribute to the pathology of cardiovascular diseases, cancer, metabolic syndrome, and aging. Crytopchrome-1 translocates to the nucleus through interaction with other clock proteins such as PER2 or ARNTL/BMAL1. The core clock genes: PER1/2/3 and CRY1/2, which are transcriptional repressors form the negative limb of the feedback loop and interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity and thereby negatively regulating their own expression. Transcription factors, CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2, form the positive limb of the feedback loop and act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes. It has been suggested that cryptochrome may act as a molecular gatekeeper to maintain CLOCK-ARNTL/BMAL1 in a poised and repressed state until the proper time for transcriptional activation. Animals with defective cryptochrome-1 display disruption in their glucose homeostasis with elevated blood glucose in response to acute feeding after an overnight fast and severely impaired glucose clearance in a glucose tolerance test.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgM in PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cryptochrome-1, clone D46-3361 Antibody, Cat. No. MABE1152, is a highly specific rat monoclonal antibody, that targets Cryptochrome-1 and has been tested in Western Blotting and Chromatin Immunoprecipitation (ChIP).
      Key Applications
      • Western Blotting
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blotting Analysis: A representative lot detected Cryptochrome-1 in Western Blotting applications (Partch, C.L., et. al. (2006). Proc Natl Acad Sci USA. 103(27):10467-72; Ye, R., et. al. (2011). J Biol Chem. 286(29):25891-902; Ozturk, N., et. al. (2009). Proc Natl Acad Sci USA. 106(8):2841-6; Chiou, Y.Y., et. al. (2016). Proc Natl Acad Sci USA. 113(41):E6072-E6079).

      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Cryptochrome-1 in Chromatin Immunoprecipitation applications (Ye, R., et. al. (2011). J Biol Chem. 286(29):25891-902).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 20 amino acids from the C-terminal region of murine Cryptochrome-1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone D46-3361 is a rat monoclonal antibody that specifically detects Cryptochrome-1 in murine species. it targets an epitope within 20 amino acids from the C-terminal region.
      IsotypeIgM
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Cry1
      UniProt Number
      Molecular Weight68 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.
      Western Blotting Analysis: A 1:500 dilution of this antibody detected Cryptochrome-1 in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1152-25UL 04054839448003