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CC03 Anti-Cyclin B1 (Ab-2) Mouse mAb (2H1-H6)

Anti-Cyclin B1 (Ab-2) Mouse mAb (2H1-H6) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
CC03
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes cyclin B1 in MCF-7 and HeLa cells.
      Catalogue NumberCC03
      Brand Family Calbiochem®
      References
      ReferencesGhiara, J.B., et al. 1991. Cell 65, 163.
      Glotzer, M., et al. 1991. Nature (London) 349, 132.
      Solomon, M.J., et al. 1990. Cell 63, 1013.
      Pines, J. 1992. Cell Growth Differ. 2, 305.
      Draetta, G., and Beach, D. 1988. Cell 54, 17.
      Minshull, J., et al. 1988. in: D. Beach, C. Basilico and J. Newport (eds). Cold Spring Harbor Laboratory 128.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Negative controltrpE (Ab-1)
      Positive controlMCF-7 or HeLa cells
      PreservativeNone
      Applications
      Key Applications Immunofluorescence
      Immunoprecipitation
      Not Western Blot
      Not Paraffin Sections
      Application NotesImmunofluorescence (2.5 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Immunoblotting (not recommended)
      Paraffin sections (not recommended)
      Application CommentsCyclin B1 (Ab-2) reacts only weakly with mouse; use with murine cyclin B1 is not recommended. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenfull-length recombinant human cyclin B1
      ImmunogenHuman
      Clone2H1-H6
      HostMouse
      IsotypeIgG₁
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C) for long-term storage.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CC03 0

      Documentation

      References

      Reference overview
      Ghiara, J.B., et al. 1991. Cell 65, 163.
      Glotzer, M., et al. 1991. Nature (London) 349, 132.
      Solomon, M.J., et al. 1990. Cell 63, 1013.
      Pines, J. 1992. Cell Growth Differ. 2, 305.
      Draetta, G., and Beach, D. 1988. Cell 54, 17.
      Minshull, J., et al. 1988. in: D. Beach, C. Basilico and J. Newport (eds). Cold Spring Harbor Laboratory 128.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-September-2007 JSW
      ApplicationImmunofluorescence (2.5 µg/ml)
      Immunoprecipitation (1 µg/sample)
      Immunoblotting (not recommended)
      Paraffin sections (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing the splenocytes with NS/1 mouse myeloma cells. Recognizes the cyclin B1 protein.
      BackgroundCyclins are a family of proteins implicated in the induction and control of mitosis. They are characterized by their accumulation and dramatic destruction in a cell cycle dependent manner. Cyclins are necessary for cdc/cdk kinase activity and are one of the subunits of the active kinase, in association with cdc2 or one of the cdk family members. In addition, the destruction of cyclin via the ubiquitin pathway is necessary for the cell to exit from mitosis. Although several different major cyclin families have been identified to date, the two major classes are the A and B cyclins. The A cyclins, identified in all eukaryotic species except for yeast, appear before the B cyclins in the course of the cell cycle. While the B type cyclins have been implicated in mitosis as well as meiosis, the exact role of the A type cyclins is still not clear.
      HostMouse
      Immunogen speciesHuman
      Immunogenfull-length recombinant human cyclin B1
      Clone2H1-H6
      IsotypeIgG₁
      Specieshuman, not mouse, not rat
      Positive controlMCF-7 or HeLa cells
      Negative controltrpE (Ab-1)
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsCyclin B1 (Ab-2) reacts only weakly with mouse; use with murine cyclin B1 is not recommended. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C) for long-term storage.
      Toxicity Standard Handling
      ReferencesGhiara, J.B., et al. 1991. Cell 65, 163.
      Glotzer, M., et al. 1991. Nature (London) 349, 132.
      Solomon, M.J., et al. 1990. Cell 63, 1013.
      Pines, J. 1992. Cell Growth Differ. 2, 305.
      Draetta, G., and Beach, D. 1988. Cell 54, 17.
      Minshull, J., et al. 1988. in: D. Beach, C. Basilico and J. Newport (eds). Cold Spring Harbor Laboratory 128.