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239784 Anti-Cyclophilin D (42-54) Rabbit pAb

Anti-Cyclophilin D (42-54) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
239784
  
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Overview

Replacement Information

Key Spec Table

Host
Rb
Description
Overview

This product has been discontinued.





Recognizes a ~15 kDa protein in rat liver and heart and a ~37 kDa protein in rat brain extract.
Catalogue Number239784
Brand Family Calbiochem®
Application Data
Detection of rat cyclophilin D by immunoblotting. Sample: Whole tissue extract from rat heart. Primary antibody: Anti-Cyclophilin D (42-54) Rabbit pAb (Cat. No. 239784) (1 µg/ml). Detection: chemiluminescence.

Detection of rat cyclophilin D by immunoblotting. Sample: Whole tissue extract from rat heart. Primary antibody: Anti-Cyclophilin D (42-54) Rabbit pAb (Cat. No. 239784) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesHalestrap, A.P., et al. 2000. Biochem. Soc. Trans. 28, 170.
Andreeva, L., et al. 1999. Int. J. Exp. Pathol. 80, 305.
Product Information
FormLiquid
FormulationIn PBS, 1 mg/ml BSA.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1:1000)
Application CommentsVariables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide [SGNPLVYLDVDAN(C)] corresponding to amino acids 42-54 of mature human cyclophilin D (also called cyclophilin F)
ImmunogenHuman
HostRabbit
IsotypeIgG
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
239784 0

Documentation

Anti-Cyclophilin D (42-54) Rabbit pAb Certificates of Analysis

TitleLot Number
239784

References

Reference overview
Halestrap, A.P., et al. 2000. Biochem. Soc. Trans. 28, 170.
Andreeva, L., et al. 1999. Int. J. Exp. Pathol. 80, 305.

Citations

Title
  • Sheng Gu, et al. (2004) Global investigation of p53-induced apoptosis through quantitative proteomic profiling using comparative amino acid-coded tagging. Molecular and Cellular Proteomics 3, 998-1003.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision26-July-2007 RFH
    ApplicationImmunoblotting (1:1000)
    Application Data
    Detection of rat cyclophilin D by immunoblotting. Sample: Whole tissue extract from rat heart. Primary antibody: Anti-Cyclophilin D (42-54) Rabbit pAb (Cat. No. 239784) (1 µg/ml). Detection: chemiluminescence.

    Detection of rat cyclophilin D by immunoblotting. Sample: Whole tissue extract from rat heart. Primary antibody: Anti-Cyclophilin D (42-54) Rabbit pAb (Cat. No. 239784) (1 µg/ml). Detection: chemiluminescence.
    DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes a ~15 kDa protein in rat liver and heart and a ~37 kDa protein in rat brain extract.
    BackgroundCyclophilin D is an immunophilin, a family of soluble cytosolic receptors capable of binding to one of two major immuno-suppressant agents, cyclosporin A (CsA) or FK506. Proteins that bind FK506 are termed FK506 Binding Proteins (FKBPs) and those that bind CsA are called cyclophilins. Immunophilins function as peptidyl prolyl cis-trans-isomerases (PPIase) whose activity is inhibited by their respective immuno-suppressant compounds. Immunophilins accelerate folding of proteins in vivo and in vitro by catalyzing slow steps in the initial folding and rearrangement of proline-containing proteins. Cyclophilin D is located in the intermembrane space of the mitochondria where it is an integral member of the permeability transition pore complex that includes the voltage dependent anion channel (VDAC) and the adenine nucleotide translocator (ANT).
    HostRabbit
    Immunogen speciesHuman
    Immunogena synthetic peptide [SGNPLVYLDVDAN(C)] corresponding to amino acids 42-54 of mature human cyclophilin D (also called cyclophilin F)
    IsotypeIgG
    Speciescanine, hamster, human, mouse, rat
    FormLiquid
    FormulationIn PBS, 1 mg/ml BSA.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsVariables associated with assay conditions will dictate the optimal working dilution.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesHalestrap, A.P., et al. 2000. Biochem. Soc. Trans. 28, 170.
    Andreeva, L., et al. 1999. Int. J. Exp. Pathol. 80, 305.
    Citation
  • Sheng Gu, et al. (2004) Global investigation of p53-induced apoptosis through quantitative proteomic profiling using comparative amino acid-coded tagging. Molecular and Cellular Proteomics 3, 998-1003.
    		•