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AP1021 Anti-Cytokeratin 18 Rabbit pAb

Anti-Cytokeratin 18 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CK18

AP1021
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~42-46 kDa cytokeratin 18 protein in MCF-7 cells.
      Catalogue NumberAP1021
      Brand Family Calbiochem®
      SynonymsAnti-CK18
      Application Data
      Detection of human cytokeratin 18 by immunoblotting. Samples: Whole cell lysate (25 µg per lane) from MCF7 cells. Primary antibody: Anti-Cytokeratin 18 Rabbit pAb (Cat. No. AP1021) (0.1 µg/ml, lane 1; 0.5 µg/ml, lane 2; 2.5 µg/ml, lane 3). Detection: chemiluminescence.
      References
      ReferencesDitzel, H., et al. 2002. J. Biol. Chem. 277, 21712-21722.
      Caulin, C., et al. 1997. J. Cell Biol. 138, 1379-1394.
      Oshima, R.G., et al. 1996. Cancer Metastasis Rev. 15, 445-471.
      Fuchs, E., and Weber, K. 1994. Annu. Rev. Biochem. 63, 345-382.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Positive controlMCF-7 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (0.1-2.5 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids near the C-terminus of human cytokeratin 18
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Mercury prohibited To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AP1021 0

      Documentation

      Anti-Cytokeratin 18 Rabbit pAb Certificates of Analysis

      TitleLot Number
      AP1021

      References

      Reference overview
      Ditzel, H., et al. 2002. J. Biol. Chem. 277, 21712-21722.
      Caulin, C., et al. 1997. J. Cell Biol. 138, 1379-1394.
      Oshima, R.G., et al. 1996. Cancer Metastasis Rev. 15, 445-471.
      Fuchs, E., and Weber, K. 1994. Annu. Rev. Biochem. 63, 345-382.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-August-2009 JSW
      SynonymsAnti-CK18
      ApplicationImmunoblotting (0.1-2.5 µg/ml)
      Application Data
      Detection of human cytokeratin 18 by immunoblotting. Samples: Whole cell lysate (25 µg per lane) from MCF7 cells. Primary antibody: Anti-Cytokeratin 18 Rabbit pAb (Cat. No. AP1021) (0.1 µg/ml, lane 1; 0.5 µg/ml, lane 2; 2.5 µg/ml, lane 3). Detection: chemiluminescence.
      DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the ~42-46 kDa cytokeratin 18 protein.
      BackgroundIntermediate filaments (IF) are classified into five groups: type I, acidic cytokeratins (CK9-CK20); type II, basic cytokeratins (CK1-CK8); type III, vimentin, desmin, GFAP, and peripherin; type IV, neurofilaments, nestin, and internexin; and type 5, lamins. Cytokeratin 8 (CK8) and cytokeratin 18 (CK18) are the major components of IFs of simple or single layer epithelial cells. During cell transformation and tumor development, the cell type specificity of CK8 and CK18 is conserved, making them useful as clinical histopathological markers. Oncogenes, which activate the Ras signal transduction pathway, stimulate CK18 gene expression through transcription factors, such as members of the ETD and AP-1 families, including jun and fos. Supporting the notion that CK18 plays a role in the tumorigenic phenotype, increased expression of CK18 has been found at the invasive front of some tumors. CK18 filaments are dramatically reorganized during apoptosis accompanied by phosphorylation on serine 53. It appears that several caspases are capable to cleave CK18 during apoptosis.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids near the C-terminus of human cytokeratin 18
      IsotypeIgG
      Specieshuman
      Positive controlMCF-7 cells
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
      Toxicity Irritant
      ReferencesDitzel, H., et al. 2002. J. Biol. Chem. 277, 21712-21722.
      Caulin, C., et al. 1997. J. Cell Biol. 138, 1379-1394.
      Oshima, R.G., et al. 1996. Cancer Metastasis Rev. 15, 445-471.
      Fuchs, E., and Weber, K. 1994. Annu. Rev. Biochem. 63, 345-382.