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MABT845-25UG Anti-Cytokeratin-20 Antibody, clone XQ1

Anti-Cytokeratin-20, clone XQ1, Cat. No. MABT845, is a mouse monoclonal antibody that detects Keratin, type I cytoskeletal 20 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-Cytokeratin-20 Antibody, clone XQ1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Krt20 UniProt Number: Q9D312
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MABT845-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIF, IH(P), WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT845-25UG
      DescriptionAnti-Cytokeratin-20 Antibody, clone XQ1
      Alternate Names
      • Keratin
      • type I cytoskeletal 20
      • CK-20
      • Keratin-20
      • K20
      Background InformationKeratin, type I cytoskeletal 20 (UniProt: Q9D312; also known as Cytokeratin-20, CK-20, Keratin-20, K20) is encoded by the Krt20 gene (Gene ID: 66809) in murine species. Cytokeratin-20 is type I keratin of the intermediate filament family and is recognized as a marker of intestinal differentiation. It is expressed at low levels in the more differentiated suprabasal regions of the small intestine, and at higher levels in the colon, mainly in the upper region and in scattered cells throughout the remaining epithelium. Cytokeratin-20 is also an excellent marker for certain types of carcinomas, such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. It plays a significant role in maintaining keratin filament organization in intestinal epithelia and when phosphorylated, it also plays a role in the secretion of mucin in the small intestine. Cytokeratin-20 can undergo hyperphosphorylation at serine 13 during the early stages of apoptosis, which becomes less prominent during the later stages. Phosphorylation at serine 13 is shown to increase in response to stress brought on by cell injury. During apoptosis it is cleaved at Asp 235 by caspases. (Ref.: Moll, R., et al. (1992). Am. J. Pathol. 140(2); 427-447).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in PBS with 0.05% sodium azide.
      Applications
      ApplicationAnti-Cytokeratin-20, clone XQ1, Cat. No. MABT845, is a mouse monoclonal antibody that detects Keratin, type I cytoskeletal 20 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected Cytokeratin-20 in Immunofluorescence applications (Zhou, Q., et. al. (2003). Mol Biol Cell. 14(7):2959-71).

      Western Blotting Analysis: A representative lot detected Cytokeratin-20 in Western Blotting applications (Zhou, Q., et. al. (2003). Mol Biol Cell. 14(7):2959-71).
      Biological Information
      ImmunogenA full-length purified murine Cytokeratin-20.
      CloneXQ1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone XQ1 is a mouse monoclonal antibody that detects cytoketatin-20 in murine tissues.
      IsotypeIgMκ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Does not react with human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Krt20
      Purification MethodPurified by ion-exchange chromatography
      UniProt Number
      Molecular Weight49.03 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse colon tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Cytokeratin-20 in mouse colon tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT845-25UG 04054839563379