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MABT1529-100UG Anti-Cytokeratin 8 Antibody, clone 35betaH11

Anti-Cytokeratin 8, clone 35betaH11, Cat. No. MABT1529, is a mouse monoclonal antibody that detects Cytokeratin-8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

MABT1529-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABT1529-100UG
      DescriptionAnti-Cytokeratin 8 Antibody, clone 35betaH11
      Alternate Names
      • Keratin
      • type II cytoskeletal 8
      • CK-8
      • Keratin-8
      • K8
      • Type-II keratin Kb8
      Background InformationKeratin, type II cytoskeletal 8 (UniProt: P05787; also known as Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8) is encoded by the KRT8 (also known as CYK8) gene (Gene ID: 3856) in human. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. Cytokeratins share a common structural organization: a central alpha-helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. CK8 is expressed in abundance in the epithelia of colon, bladder, ileum, and stomach. It is a type II, neutral to basic protein that together with cytokeratin-19 (KRT19) helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-8 can undergo phosphorylation on three major serine residues: Serine 23, 431, and 73. Serine 23 is shown to be highly conserved in all type II keratins. Phosphorylation at Serine 73 is reported to increase during cellular stress. However, under normal conditions serine 73 remains largely dephosphorylated. It can also undergo O-glycosylation in a cell cycle-dependent manner and glycosylation increases its solubility and reduces stability by inducing proteasomal degradation. CK8 expression has been correlated with malignancy in leukoplakia and carcinomas of the head and neck and its expression has been observed in all non-small-cell lung cancers.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgM in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Cytokeratin 8, clone 35betaH11, Cat. No. MABT1529, is a mouse monoclonal antibody that detects Cytokeratin-8 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected Cytokeratin-8 in human prostate tissue sections.

      Western Blotting Analysis: A representative lot detected Cytokeratin-8 in Western Blotting applications (Gown, A.M., et. al. (1984). Am J Pathol. 114(2):309-21).

      Immunohistochemistry (Paraffin) Analysis: A representative lot detected Cytokeratin-8 in Immunohistochemistry applications (Gown, A.M., et. al. (1984). Am J Pathol. 114(2):309-21).
      Biological Information
      ImmunogenA cytoskeletal preparation of the human hepatocellular carcinoma cell line Hep3B.
      Clone35betaH11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 35betaH11 is a mouse monoclonal antibody that specifically detetcts human Cytokeratin 8.
      IsotypeIgMκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KRT8
      • CYK8
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight53.70 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human pancreas tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected Cytokeratin-8 in human pancreas tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT1529-100UG 04054839997358

      Documentation

      Anti-Cytokeratin 8 Antibody, clone 35betaH11 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cytokeratin 8 Antibody, clone 35betaH11 Certificates of Analysis

      TitleLot Number
      Anti-Cytokeratin 8, clone 35betaH11 - Q3236794 Q3236794

      References

      Reference overviewPub Med ID
      Monoclonal antibodies to human intermediate filament proteins. II. Distribution of filament proteins in normal human tissues.
      Gown, AM; Vogel, AM
      Am J Pathol  114  309-21  1984

      Show Abstract       		6320650 6320650