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MABT329 Anti-Cytokeratin 8 Antibody, clone TROMA-1

This rat monoclonal Anti-Cytokeratin 8, clone TROMA-1, Cat. No. MABT329, is a rat monoclonal antibody that detects cytokeratin-8 in human and murine cells & is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Radioimmunoassay, & WB.

Anti-Cytokeratin 8 Antibody, clone TROMA-1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: Krt8, Krt2-8 UniProt Number: P11679
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MABT329
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, ICC, IH(P), RIA, IF, IPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABT329
      DescriptionAnti-Cytokeratin 8 Antibody, clone TROMA-1
      Alternate Names
      • Keratin type II cytoskeletal 8
      • Cytokeratin endo A
      • Cytokeratin-8
      • CK-8
      • Keratin-8
      • K8
      • Type-II keratin Kb8
      Background InformationKeratin, type II cytoskeletal 8 (UniProt: P11679; also known as Cytokeratin endo A, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8) is encoded by the Krt8 (also known as Krt2-8) gene (Gene ID: 16691) in murine species. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. Cytokeratins share a common structural organization: a central a-helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. CK8 is a heterotetramer of two type I and two type II keratins. It is expressed in abundance in the epithelia of colon, bladder, ileum, and stomach. CK8 together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK8 is often used together with keratin 18 and keratin 19 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood. CK8 expression has been correlated with malignancy in leukoplakia and carcinomas of the head and neck and its expression has been observed in all non-small-cell lung cancers.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rat monoclonal Anti-Cytokeratin 8, clone TROMA-1, Cat. No. MABT329, is a rat monoclonal antibody that detects cytokeratin-8 in human and murine cells & is tested for use in Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, Radioimmunoassay, & WB.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Radioimmunoassay
      • Immunofluorescence
      • Immunoprecipitation
      Application NotesImmunocytochemistry Analysis: A representative lot detected Cytokeratin 8 in Immunocytochemistry applications (O'Driscoll, C.M., et. al. (2013). Biochim Biophys Acta. 1833(3):460-7; Wang, X., et. al. (2004). J Cell Sci. 117(Pt 1):53-62).

      Western Blotting Analysis: A representative lot detected Cytokeratin 8 in Western Blotting applications (Helenius, T.O., et. al. (2016). Cells. 5(3); O'Driscoll, C.M., et. al. (2013). Biochim Biophys Acta. 1833(3):460-7; Tao, G.Z., et. al. (2003). J Cell Sci. 116(Pt 22):4629-38; Maurer, J., et. al. (2008) PLoS One. 3(10):e3451).

      Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected Cytokeratin 8 in HepG2 cells.

      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cytokeratin 8 in mouse lung tissue.

      Immunoprecipitation Analysis: A representative lot detected Cytokeratin 8 in Immunoprecipitation applications (Brulet, P., et. al. (1982). Proc Natl Acad Sci USA. 79(7):2328-32).

      Radioimmunoassay Analysis: A representative lot detected Cytokeratin 8 in Radioimmunoassy applications (Brulet, P., et. al. (1982). Proc Natl Acad Sci USA. 79(7):2328-32).

      Immunofluorescence Analysis: A representative lot detected Cytokeratin 8 in Immunofluorescence applications (Helenius, T.O., et. al. (2016). Cells. 5(3); Tao, G.Z., et. al. (2003). J Cell Sci. 116(Pt 22):4629-38; Lin, Y.M., et. al. (2008). J Cell Sci. 121(Pt 14):2382-93; Maurer, J., et. al. (2008) PLoS One. 3(10):e3451; Nuotio-Antar, A.M., et. al. (2006). J Steroid Biochem Mol Biol. 99(2-3):93-9; Tamai, Y., et. al. (2000). J Cell Biol. 151(3):563-72).

      Immunohistochemistry Analysis: A representative lot detected Cytokeratin 8 in Immunohistochemistry applications (Szeder, V., et. al. (2003). Dev Biol. 253(2):258-63; Tao, G.Z., et. al. (2003). J Cell Sci. 116(Pt 22):4629-38; Nuotio-Antar, A.M., et. al. (2006). J Steroid Biochem Mol Biol. 99(2-3):93-9; Tamai, Y., et. al. (2000). J Cell Biol. 151(3):563-72).
      Biological Information
      ImmunogenIntermediate filaments extracted from TDM1 Trophoblastoma cells.
      CloneTROMA-1
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone TROMA-1 detects cytokeratin-8 in human and murine cells.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Krt8
      • Krt2-8
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~54 kDa observed; 54.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 0.5 ug/mL of this antibody detected Cytokeratin 8 in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT329 04054839322501

      Documentation

      Anti-Cytokeratin 8 Antibody, clone TROMA-1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cytokeratin 8 Antibody, clone TROMA-1 Certificates of Analysis

      TitleLot Number
      Anti-Cytokeratin 8, clone TROMA-1 - 3212295 3212295
      Anti-Cytokeratin 8, clone TROMA-1 - 3321789 3321789
      Anti-Cytokeratin 8, clone TROMA-1 - 3429042 3429042
      Anti-Cytokeratin 8, clone TROMA-1 - 3738424 3738424
      Anti-Cytokeratin 8, clone TROMA-1 - 3768051 3768051
      Anti-Cytokeratin 8, clone TROMA-1 - 3799999 3799999
      Anti-Cytokeratin 8, clone TROMA-1 - 3840336 3840336
      Anti-Cytokeratin 8, clone TROMA-1 - 3872841 3872841
      Anti-Cytokeratin 8, clone TROMA-1 - 3885482 3885482
      Anti-Cytokeratin 8, clone TROMA-1 - 3917378 3917378
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