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ABE1950 Anti-DBC1 Antibody

Detect DBC1 using this Anti-DBC1 Antibody validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP).

Anti-DBC1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: CCAR2, DBC1, KIAA1967 UniProt Number: Q8N163
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ABE1950
200 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ICC, IHC, ChIPRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1950
      DescriptionAnti-DBC1 Antibody
      Alternate Names
      • Cell cycle and apoptosis regulator protein 2
      • Cell division cycle and apoptosis regulator protein 2
      • DBIRD complex subunit KIAA1967
      • Deleted in breast cancer gene 1 protein
      • DBC-1
      • DBC.1
      • p30 DBC
      • DBC1
      Background InformationCell cycle and apoptosis regulator protein 2 (UniProt Q8N163; also known as Cell division cycle and apoptosis regulator protein 2, DBC-1, DBC.1, DBIRD complex subunit KIAA1967, Deleted in breast cancer 1, p30 DBC, p30 DBC protein) is encoded by the CCAR2 (also known as DBC1, KIAA1967, NET35) gene (Gene ID 57805) in human. Deleted in breast cancer gene 1 protein (DBC1) is a nuclear protein involved in regulating functions of nuclear receptors (NRs), including androgen receptor (AR) and estrogen receptor (ER) alpha and beta. DBC1 stabilizes the interaction between chicken ovalbumin upstream promoter transcription factor I (COUP-TFI) and NCoR by interacting directly with both proteins. DBC1 also interacts with NR complex interacting factor (NRC)-1 to drive the transcription regulation of NRs. Caspase-dependent processing of DBC1 promotes apoptosis, and depletion of DBC1 negatively regulates p53-dependent apoptosis through its specific inhibition of SIRT1. The N-terimus of DBC1 is shown to interact with BRCA1 C-terminal BRCT domain and the expression of DBC1 represses BRCA1-mediated transactivation of the SIRT1 promoter. DBC1 is also a negative regulator against the ligand-dependent transcriptional activation function of ERbeta. The DBC1 protein encoded by the CCAR2 gene is not the same as the protein encoded by the BRINP1 gene, which also goes by the same acronym DBC1 for "Deleted in bladder cancer protein 1".
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect DBC1 using this Anti-DBC1 Antibody validated for use in Western Blotting, Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Chromatin Immunoprecipitation (ChIP).
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesWestern Blotting Analysis: A representative lot detected DBC1 in HeLa cell lysate (Hiraike, H., et al. (2010). British Journal of Cancer. 102:1061-1067).
      Western Blotting Analysis: A representative lot detected DBC1 in MDA-MB-231 cell lysate (Koyama, S., et al. (2010). BBRC. 392:357-362).
      Immunoprecipitation Analysis: A representative lot immunoprecipitated DBC1 in HeLa cell lysate (Hiraike, H., et al. (2010). British Journal of Cancer. 102:1061-1067).
      Immunocytochemistry Analysis: A representative lot detected DBC1 in MCF7 cells (Hiraike, H., et al. (2010). British Journal of Cancer. 102:1061-1067).
      Immunocytochemistry Analysis: A representative lot detected DBC1 in MCF7 and T47D cells (Koyama, S., et al. (2010). BBRC. 392:357-362).
      Immunohistochemistry Analysis: A representative lot detected DBC1 in ductal epithelium and adipose regions of human breast tissue sections (Hiraike, H., et al. (2010). British Journal of Cancer. 102:1061-1067).
      Immunohistochemistry Analysis: A representative lot detected DBC1 in human breast cancer tissue (Hiraike, H., et al. (2011). Experimental and Therapeutic Medicane. 2:1105-1109).
      Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected DBC1 occupancy at the SIRT1 gene promoter region (1354–1902) known to be targeted by BRCA1 (Hiraike, H., et al. (2010). Br. J. Cancer. 102(6):1061-1067).
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to human DBC1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityExpected to reacts with both spliced isoforms.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CCAR2
      • DBC1
      • KIAA1967
      Purification MethodProtein A Purfied
      UniProt Number
      Molecular Weight~130 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in MCF7 cell lysate.

      Western Blotting Analysis: 2.0 µg/mL of this antibody detected DBC1 in 10 µg of MCF7 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size200 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1950 04055977173062

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies