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MABF2189-100UG Anti-Dengue Virus 4 NS1 Antibody, clone 626

Anti-Dengue Virus 4 NS1, clone 626, Cat. No. MABF2189, is a mouse monoclonal antibody that detects Dengue virus 4 NS1 protein and is tested for use in ELISA and Flow Cytometry.

MABF2189-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2189-100UG
      DescriptionAnti-Dengue Virus 4 NS1 Antibody, clone 626
      Alternate Names
      • DENV 4 NS1 protein
      • Dengue virus 4 NS1 protein
      Background InformationDengue virus (DENV) is a mosquito-borne flavivirus and is one of the most common mosquito-transmitted viral infections. The DENV particle is about 500 nm in diameter and includes a positive-sense RNA genome with ~10,700 nucleotides and 3 structural proteins known as capsid (C), precursor membrane (prM), and envelope (E) proteins. In addition, the RNA genome of dengue virus also encodes seven nonstructural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5) that are shown to be essential for viral replication. DENV NS1, a 48-kDa glycoprotein (aa 776-1127 of genome polyprotein), is highly conserved among all flaviviruses and is essential for viral replication. It is expressed as a monomer in infected cells and after post-translational modification in the lumen of endoplasmic reticulum, it forms homodimers associated with organelle membranes and the cell membrane. NS1 is reported to be involved in immune evasion, pathogenesis, and viral replication. Once cleaved off the polyprotein, is targeted to three destinations: the viral replication cycle, the plasma membrane and the extracellular compartment. NS1 is secreted in a hexamer form, which is composed of three dimers with a detergent-sensitive hydrophobic central cavity and it plays a role against host immune response. NS1 is shown to induce expression of sialidases, heparanase, and activates cathepsin L, which activates heparinase via enzymatic cleavage. These effects are may contribute to the endothelial hyperpermeability commonly observed in severe dengue disease. During the recovery phase, NS1 is cleared from the circulation by antibody-mediated effects. Clone 626 displays high specificity for DENV 4 NS1. (Ref.: Chen, RU et al. (2018). J Biomed Sci. 25(1); 58; Bosch, I., et al. (2017). Sci. Transl. Med. 9(409); eaan1589).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Dengue Virus 4 NS1, clone 626, Cat. No. MABF2189, is a mouse monoclonal antibody that detects Dengue virus 4 NS1 protein and is tested for use in ELISA and Flow Cytometry.
      Key Applications
      • ELISA
      • Flow Cytometry
      Application NotesELISA Analysis: A representative lot detected Dengue Virus 4 NS1 in ELISA applications (Bosch, I., et. al. (2017). Sci Transl Med. 9(409); eaan1589).

      Flow Cytometry Analysis: A representative lot detected Dengue Virus 4 NS1 in Flow Cytometry applications (Bosch, I., et. al. (2017). Sci Transl Med. 9(409); eaan1589).
      Biological Information
      ImmunogenPurified recombinant Dengue virus NS1 protein expressed in mammalian cells.
      Clone626
      HostMouse
      SpecificityClone 626 is a mouse monoclonal antibody that detects DENV 4 NS1 protein. It targets an epitope within the wing/finger and beta-ladder domains.
      IsotypeIgG2bκ
      Species Reactivity
      • Virus
      Species Reactivity NoteVirus (Dengue virus).
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~48 kDa calculated for NS1 protein; ~378.38 kDa for the entire genome polyprotein.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by ELISA in DENV4 NS1 recombinant protein.

      ELISA Analysis: 5 µg/mL of this antibody detected Dengue Virus 4 NS1 recombinant protein.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2189-100UG 04061841588474

      Documentation

      Anti-Dengue Virus 4 NS1 Antibody, clone 626 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Dengue Virus 4 NS1 Antibody, clone 626 Certificates of Analysis

      TitleLot Number
      Anti-Dengue Virus 4 NS1, clone 626 - Q3420065 Q3420065

      References

      Reference overviewPub Med ID
      Topological mapping of neutrophil cytochrome b epitopes with phage-display libraries.
      Burritt, JB; Quinn, MT; Jutila, MA; Bond, CW; Jesaitis, AJ
      J Biol Chem  270  16974-80  1995

      Show Abstract       		7622517 7622517