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ABE2587-25UL Anti-Dyskerin (Rabbit)

Anti-Dyskerin (Rabbit), Cat. No. ABE2587-25UL, is a highly specific rabbit polyclonal antibody, that targets H/ACA ribonucleoprotein complex subunit 4 and has been tested in Immunoprecipitation, Immunofluorescence and Western Blotting.

Anti-Dyskerin (Rabbit) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: DKC1, NOLA4 UniProt Number: O60832
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ABE2587-25UL
25 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, IF, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE2587-25UL
      DescriptionAnti-Dyskerin (Rabbit)
      Alternate Names
      • H/ACA ribonucleoprotein complex subunit 4
      • CBF5 homolog
      • Nopp140-associated protein of 57 kDa
      • Nucleolar protein NAP57
      • Nucleolar protein family A member 4
      • snoRNP protein DKC1
      Background InformationH/ACA ribonucleoprotein complex subunit 4 (UniProt: O60832; also known as CBF5 homolog, Dyskerin, Nopp140-associated protein of 57 kDa, Nucleolar protein NAP57, Nucleolar protein family A member 4, snoRNP protein DKC1) is encoded by the DKC1 (also known as NOLA4) gene (Gene ID: 1736) in human. Dyskerin is a highly conserved nucleolar protein that is involved with multiple cellular functions, such as protein expression, growth, proliferation, and nucleo-cytoplasmic shuttling. It helps in the stabilization of the telomerase RNA component, which allows for the proper functioning of telomerase enzymatic complex. It also modifies specific uridine residues of ribosomal RNA by converting them to pseudouridine, thus allowing proper ribosome processing and function. Point mutations in the DKC1 gene result in a rare inherited syndrome called X-linked dyskeratosis congenita, which is leads to early mortality due to progressive bone marrow failure and is characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. (Ref.: Montanaro, L (2010). J. Pathol. 222(4): 345-9).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 20 mM Tris, 200 mM KCl, 1 mM EDTA, pH 8.5 with 0.02% sodium azide.
      Applications
      ApplicationAnti-Dyskerin (Rabbit), Cat. No. ABE2587-25UL, is a highly specific rabbit polyclonal antibody, that targets H/ACA ribonucleoprotein complex subunit 4 and has been tested in Immunoprecipitation, Immunofluorescence and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected Dyskerin in  10 µg of HeLa cell lysate.
      Immunoprecipitation Analysis: A representative lot detected Dyskerin in telomerase-overexpressing HEK293T cells (C ourtesy of the laboratories of Dr. Hilda Pickett and Dr. Tracy Bryan, Children’s Medical Research Institute, Australia ).
      Western Blotting Analysis: A representative lot detected Dyskerin in Western Blotting applications (O'Brien, R., et. al. (2016). Cancer Res. 76(12):3604-17).
      Western Blotting Analysis: A representative lot detected Dyskerin in dyskerin knockdown in mid-S phase HEK-293T cells (Moye, A.L., et. al. (2015). Nat Commun. 6:7643).
      Immunofluorescence Analysis: A representative lot detected Dyskerin in HeLa ceells (telomerase positive) (C ourtesy of the laboratories of Dr. Hilda Pickett and Dr. Tracy Bryan, Children’s Medical Research Institute, Australia ).
      Western Blotting Analysis: A representative lot detected Dyskerin in HEK293 Cell lysate and HEK293 cells treated with siRNA against Dyskerin (C ourtesy of the laboratories of Dr. Hilda Pickett and Dr. Tracy Bryan, Children’s Medical Research Institute, Australia ).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-teminal half of human Dyskerin, isoform 1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Dysterin in human cells. it targets and epitope within 16 amino acids from the C-terminal half. For immunoprecipitaion the use of HEPES or Tris bufer with KCl is highly recommended.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Gene Symbol
      • DKC1
      • NOLA4
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight62 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in SW480 cell lysate.
      Western Blotting Analysis: A 1:500 dilution of this antibody detected Dyskerin in  10 µg of SW480 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2587-25UL 04054839464713