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04-087 Anti-E2F-6 Antibody, clone TFE61

Anti-E2F-6 Antibody, clone TFE61 is a Mouse Monoclonal Antibody for detection of E2F-6 also known as E2F transcription factor 6 & has been validated in WB, IP, ICC.

Anti-E2F-6 Antibody, clone TFE61 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors, Cell Cycle, DNA Replication & Repair Gene Symbol: E2F-6, E2F6 UniProt Number: O75461
04-087
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IP, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-087
      DescriptionAnti-E2F-6 Antibody, clone TFE61
      Alternate Names
      • E2F transcription factor 6
      • E2F transcription factor 6, isoform 1
      Background InformationThe E2F family of transcription factors includes the E2Fs and DP1. These proteins are critical regulators of genes required for cell cycle progression, and their activity is regulated by association with different protein partners during stages of the cell cycle. E2Fs bind the hypophosphorylated form of pRb, and are maintained in an inactive state. Phosphorylation of Rb causes dissociation, and activation of E2F activity.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-E2F-6 Antibody, clone TFE61 is a Mouse Monoclonal Antibody for detection of E2F-6 also known as E2F transcription factor 6 & has been validated in WB, IP, ICC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      Biological Information
      ImmunogenFull-length recombinant human E2F-6 protein
      EpitopeFull-length
      CloneTFE61
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody detects human E2F-6.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes a member of the E2F transcription factor protein family. E2F family members play a crucial role in control of the cell cycle and of the action of tumor suppressor proteins. They are also a target of the transforming proteins of small DNA tumor viruses. Many E2F proteins contain several evolutionarily conserved domains: a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. The encoded protein of this gene is atypical because it lacks the transactivation and tumor suppressor protein association domains. It contains a modular suppression domain and is an inhibitor of E2F-dependent transcription. The protein is part of a multimeric protein complex that contains a histone methyltransferase and the transcription factors Mga and Max. Multiple transcript variants have been reported for this gene, but it has not been clearly demonstrated that they encode valid isoforms. [provided by RefSeq]
      Gene Symbol
      • E2F-6
      • E2F6
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Inhibitor of E2F-dependent transcription. Binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3'. Has a preference for the 5'-TTTCCCGC-3' E2F recognition site. E2F-6 lacks the transcriptional activation and pocket protein binding domains. Appears to regulate a subset of E2F-dependent genes whose products are required for entry into the cell cycle but not for normal cell cycle progression. May silence expression via the recruitment of a chromatin remodeling complex containing histone H3-K9 methyltransferase activity. Overexpression delays the exit of cells from the S-phase.
      SUBUNIT STRUCTURE: Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Expressed in all tissues examined. Highest levels in placenta, skeletal muscle, heart, ovary, kidney, small intestine and spleen.
      SEQUENCE SIMILARITIES: Belongs to the E2F/DP family.
      Molecular Weight~35-38 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by western blot on HeLa nuclear extracts.

      Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect E2F-6 in HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      04-087 04053252582172