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DR1095 Anti-E2F2 Rabbit pAb

Anti-E2F2, rabbit polyclonal, recognizes the ~40-45 kDa E2F2 protein in HeLa cells and in mouse kidney. It is validated for use in Western blotting, and immunocytochemistry.

Anti-E2F2 Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-E2F Transcription Factor 2

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DR1095
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MRbPolyclonal Antibody

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DR1095-100UG
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      Description
      OverviewRecognizes the ~40-45 kDa E2F2 protein in HeLa cells and mouse kidney tissue.
      Catalogue NumberDR1095
      Brand Family Calbiochem®
      SynonymsAnti-E2F Transcription Factor 2
      Application Data
      Detection of mouse E2F2 by immunoblotting. Sample: Whole tissue extract from mouse kidney. Primary antibody: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human E2F2 by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1:1200) and an Anti-E2F2 Mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlMouse kidney tissue, HeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1-5 µg/ml)
      Immunocytochemistry (1:1200)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, human E2F2 (aa 1-437)
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1095-100UG 04055977226140

      Documentation

      Anti-E2F2 Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-E2F Transcription Factor 2
      ApplicationImmunoblotting (1-5 µg/ml)
      Immunocytochemistry (1:1200)
      Application Data
      Detection of mouse E2F2 by immunoblotting. Sample: Whole tissue extract from mouse kidney. Primary antibody: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human E2F2 by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1:1200) and an Anti-E2F2 Mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain.
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~40-45 kDa E2F2 protein.
      BackgroundThe E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds specifically to retinoblastoma protein in a cell-cycle dependent manner, and it exhibits overall 46% amino acid identity to E2F1.
      HostRabbit
      Immunogen speciesHuman
      ImmunogenFull-length, human E2F2 (aa 1-437)
      IsotypeIgG
      Specieshuman, mouse
      Positive controlMouse kidney tissue, HeLa cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review