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DR1055 Anti-EIF2S1 Mouse mAb (3H12-C11)

Anti-EIF2S1 Mouse mAb (3H12-C11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Eukaryotic Translation Initiation Factor2,1α Subunit

DR1055
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~37 kDa EIF2S1 protein in HeLa NE cells.
      Catalogue NumberDR1055
      Brand Family Calbiochem®
      SynonymsAnti-Eukaryotic Translation Initiation Factor2,1α Subunit
      Application Data
      Detection of human EIF2S1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-EIF2S1 Mouse mAb (3H12-C11) (Cat. No. DR1055) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human EIF2S1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-EIF2S1 Mouse mAb (3H12-C11) (Cat. No. DR1055) (10 µg/ml). Detection: fluorescence.
      References
      ReferencesWeinlich, S., et al. 2009. RNA 15, 1528.
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlHeLa NE cells
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution
      Biological Information
      ImmunogenFull-length, recombinant human EIF2S1 (aa 1-316), expressed as a GST fusion protein
      Clone3H12-C11
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1055 0

      Documentation

      References

      Reference overview
      Weinlich, S., et al. 2009. RNA 15, 1528.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-Eukaryotic Translation Initiation Factor2,1α Subunit
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human EIF2S1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-EIF2S1 Mouse mAb (3H12-C11) (Cat. No. DR1055) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human EIF2S1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-EIF2S1 Mouse mAb (3H12-C11) (Cat. No. DR1055) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~37 kDa EIF2S1 protein.
      BackgroundThe translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits.
      HostMouse
      ImmunogenFull-length, recombinant human EIF2S1 (aa 1-316), expressed as a GST fusion protein
      Clone3H12-C11
      IsotypeIgG₁
      Specieshuman
      Positive controlHeLa NE cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review
      ReferencesWeinlich, S., et al. 2009. RNA 15, 1528.