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ABE2596-100UG Anti-ENL (MLLT1)

Anti-ENL (MLLT1), Cat. No. ABE2596, is a rabbit polyclonal antibody that detects protein ENL (MLLT1) and is tested for use in ChIP-seq, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

ABE2596-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABE2596-100UG
      DescriptionAnti-ENL (MLLT1)
      Alternate Names
      • Protein ENL
      • YEATS domain-containing protein 1
      Background InformationProtein ENL (UniProt: Q03111; also known as YEATS domain-containing protein 1, MLLT1) is encoded by the MLLT1 (also known as ENL, LTG19, YEATS1) gene (Gene ID: 4298) in human. ENL is a component of the super elongation complex that is required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. It displays high homology with AF9/MLLT3 in its N-terminal YEATS domain and both are shown to be fusion partners of Mixed Lineage Leukemia (MLL), TrxG proteins. In the super elongation complex they connect it to other transcriptional factors and regulate transcriptional elongation. In response to double strand breaks both ENL and AF9 in the super elongation complex are phosphorylated by ATM, which increases the interaction between ENL in this complex and BMI1/RING1B in PRC1. The phosphorylated ENL recruits PRC1 at transcriptional elongation sites leading to the ubiquitination of histone H2A and results in transcriptional repression. Mutations in its YEATS domain have been linked to Wilms tumors. The mutant MLLT1 gene exhibits altered binding to acetylated histone tails and increased expression of Myc gene. A chromosomal aberration involving MLLT1 has also been reported in acute leukemias. (Ref.: Moustakim, M., et al., (2018). Angew Chem. Int. Ed. Engl. 57(50); 16302-16307; Ui, A., and Yasui, A. (2016). Nucleus. 7(2); 138-145; Pearlman, EJ., et al. (2015). Nat. Commun. Nat Commun. 6; 10013).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-ENL (MLLT1), Cat. No. ABE2596, is a rabbit polyclonal antibody that detects protein ENL (MLLT1) and is tested for use in ChIP-seq, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • ChIP-seq
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Western Blotting
      Application NotesImmunocytochemistry Analysis : A 1:1,000 dilution from a representative lot detected ENL (MLLT1) in HeLa cells.

      Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected ENL (MLLT1) in human cerebellum tissue sections.

      ChIP-seq Analysis: 20 mg (1 mg/mL) from a representative lot detected ENL (MLLT1) in Human Leukemia MOLM-13 expressing Flag-ENL (Data courtesy of Dr. Liling Wan and Dr. C. David Allis, The Rockefeller University).


      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to 105 amino acids from the C-terminal half of human ENL (MLLT1) protein.
      Epitopeunknown
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects human ENL (MLLT1) protein. It targets an epitope within the C-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MLLT1
      • ENL
      • LTG19
      • YEATS1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight62.06 kDa calculated.. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293-Flag-ENL transgene overexpression.

      Western Blotting Analysis:A 1:1,000 dilution of this antibody detected ENL (MLLT1) protein in lysate from HEK293 cells overexpressing Flag-ENL (MLLT1).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE2596-100UG 04061841990222