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CA1019 Anti-EPLIN, Internal Rabbit pAb

Anti-EPLIN, Internal Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Epithelial Protein Lost in Neoplasm

CA1019
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~67 kDa α and the ~85 kDa β isoforms of EPLIN in OU145 cells.
      Catalogue NumberCA1019
      Brand Family Calbiochem®
      SynonymsAnti-Epithelial Protein Lost in Neoplasm
      Application Data
      Detection of human EPLIN by immunoprecipitation. Sample: Whole cell lysate from DU145 cells. Antibody for immunoprecipitation: Anti-EPLIN, Internal Rabbit pAb (Cat. No. CA1019) (10 µg/mg total protein). Antibody for immunoblotting: Anti-EPLIN C-Terminal Rabbit pAb (Cat. No. CA1014) (1:1000). Detection: chemiluminescence.
      References
      ReferencesMaul, R.S., et al. 2003. J. Cell Biol. 160, 399.
      Chen, S., et al. 2000. Gene 248, 69.
      Maul, R.S., et al. 1999. Oncogene 18, 7838.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Positive controlDU145 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoprecipitation
      Not Western Blot
      Application NotesImmunoprecipitation (10 µg/mg total protein)
      Immunoblotting (not recommended, see comments)
      Application CommentsFor immunoblotting, Anti-EPLIN, Human (Rabbit), Cat. No. CA1014 is recommended. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids from an internal region of human EPLIN
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Highly Toxic
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CA1019 0

      Documentation

      References

      Reference overview
      Maul, R.S., et al. 2003. J. Cell Biol. 160, 399.
      Chen, S., et al. 2000. Gene 248, 69.
      Maul, R.S., et al. 1999. Oncogene 18, 7838.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision20-August-2007 RFH
      SynonymsAnti-Epithelial Protein Lost in Neoplasm
      ApplicationImmunoprecipitation (10 µg/mg total protein)
      Immunoblotting (not recommended, see comments)
      Application Data
      Detection of human EPLIN by immunoprecipitation. Sample: Whole cell lysate from DU145 cells. Antibody for immunoprecipitation: Anti-EPLIN, Internal Rabbit pAb (Cat. No. CA1019) (10 µg/mg total protein). Antibody for immunoblotting: Anti-EPLIN C-Terminal Rabbit pAb (Cat. No. CA1014) (1:1000). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~67 kDa α and the ~85 kDa β isoforms of EPLIN.
      BackgroundEPLIN (Epithelial Protein Lost in Neoplasm, Accesion No. NP 057441) was originally identified as the product of a gene that is down-regulated in prostate and breast cancer cell lines. In humans, EPLIN is expressed as two isoforms, EPLIN-α (~67 kDa) and EPLIN-β (~85 kDa), as a result of alternative promoter usage from a single gene on chromosome 12. The EPLIN-β isoform differs only from the EPLIN-α isoform by an additional 160 amino acids at the N-terminus. EPLIN-α expression is preferentially down-regulated in transformed cells. EPLIN contains a LIM domain that may facilitate dimerization and protein association. Expression of EPLIN increases the assembly of cellular stress fibers and inhibits membrane ruffling induced by active Rac. EPLIN stabilizes preformed filaments through its ability to interact with and bundle actin filaments. Loss of EPLIN may contribute to transformation due to rapid turnover of filaments associated with motility.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids from an internal region of human EPLIN
      IsotypeIgG
      Specieshuman
      Positive controlDU145 cells
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsFor immunoblotting, Anti-EPLIN, Human (Rabbit), Cat. No. CA1014 is recommended. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Toxicity Highly Toxic
      ReferencesMaul, R.S., et al. 2003. J. Cell Biol. 160, 399.
      Chen, S., et al. 2000. Gene 248, 69.
      Maul, R.S., et al. 1999. Oncogene 18, 7838.