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ST1118 Anti-ESPL1/Separase Rabbit pAb

Anti-ESPL1/Separase Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ST1118
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~220 kDa ESPL1/separase protein in HepG2 cells.
      Catalogue NumberST1118
      Brand Family Calbiochem®
      Application Data
      Lane 1: Detection of human separase by immunoblotting. Sample: Whole cell lysate (20 µg) from HepG2 cells. Primary antibody: Anti-Separase Rabbit pAb (Cat. No. ST1118) (1 µg/ml). Detection: chemiluminescence. Lane 2: Detection of human separase by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HepG2 cells. Antibody for immunoprecipitation: Anti-Separase Rebbit pAb (Cat no. ST1118) (5 µg/500 µg total protein). Immunoblotting conditions: Sample: half of the immunoprecipitated separase. Primary antibody: Anti-Separase Rabbit pAb (Cat No. ST1118) (2 µg/ml). Detection: chemiluminescence.
      References
      ReferencesGorr, I.H., et al. 2006. Nat. Cell. Biol. 8, 1035.
      Holland, A.J. and Taylor, S.S. 2006. J. Cell Sci. 119, 3325.
      Kudo, N.R., et al. 2006. Cell 126, 135.
      Product Information
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Positive controlHepG2 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesImmunoblotting (1-2 µg/ml)
      Immunoprecipitation (10 µg/mg total protein)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids in the middle region of human separase.
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ST1118 0

      Documentation

      References

      Reference overview
      Gorr, I.H., et al. 2006. Nat. Cell. Biol. 8, 1035.
      Holland, A.J. and Taylor, S.S. 2006. J. Cell Sci. 119, 3325.
      Kudo, N.R., et al. 2006. Cell 126, 135.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-October-2007 RFH
      ApplicationImmunoblotting (1-2 µg/ml)
      Immunoprecipitation (10 µg/mg total protein)
      Application Data
      Lane 1: Detection of human separase by immunoblotting. Sample: Whole cell lysate (20 µg) from HepG2 cells. Primary antibody: Anti-Separase Rabbit pAb (Cat. No. ST1118) (1 µg/ml). Detection: chemiluminescence. Lane 2: Detection of human separase by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 µg) from HepG2 cells. Antibody for immunoprecipitation: Anti-Separase Rebbit pAb (Cat no. ST1118) (5 µg/500 µg total protein). Immunoblotting conditions: Sample: half of the immunoprecipitated separase. Primary antibody: Anti-Separase Rabbit pAb (Cat No. ST1118) (2 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~220 kDa ESPL1/separase protein.
      BackgroundSeparase (accession no. NP_036423) is a ~220 kDa cysteine protease essential for both mitosis and meiosis. Cancer cells often have abnormal chromosome numbers, indicating that abberant chromosomal division contributes to cancer development. During cell division, the sister chromatids are held together by the protein, cohesin. During anaphase, separase cleaves cohesin, releasing the sister chromatids. Separse is also essential during meiosis I exit, which is not dependent on the proteolytic activity of separase, but its phosphorylation at Ser1126. Phosphorylated separase forms a complex with Cdk1, which inhibits both the protease and kinase activities, respectively, of the two components, and signals the end of meiosis I.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids in the middle region of human separase.
      IsotypeIgG
      Specieshuman
      Positive controlHepG2 cells
      FormLiquid
      FormulationIn Tris-citrate/phosphate buffer, pH 7.0-8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesGorr, I.H., et al. 2006. Nat. Cell. Biol. 8, 1035.
      Holland, A.J. and Taylor, S.S. 2006. J. Cell Sci. 119, 3325.
      Kudo, N.R., et al. 2006. Cell 126, 135.