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MABC681 Anti-ETV4 Antibody, clone 1A2G3

Detect ETS translocation variant 4 protein using this mouse monoclonal antibody, Anti-ETV4 Antibody & clone 1A2G3 validated for use in western blotting.

Anti-ETV4 Antibody, clone 1A2G3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: ETV4, E1AF, PEA3 UniProt Number: P43268
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MABC681
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABC681
      DescriptionAnti-ETV4 Antibody, clone 1A2G3
      Alternate Names
      • ETS translocation variant 4
      • Adenovirus E1A enhancer-binding protein
      • E1A-F
      • Polyomavirus enhancer activator 3 homolog
      • Protein PEA3
      Background InformationETS translocation variant 4 protein, alternatively known as Adenovirus E1A enhancer-binding protein E1A-F, or Polyomavirus enhancer activator 3 homolog (PEA3), is encoded by the human gene named ETVA/E1AF/PEA3. ETV4 is a protein that is a transcriptional activator that was first recognized as such because it allowed for the expression of the adenovirus E1A gene upon infection. Today ETV4 has been shown to be active in a wide variety of biological processes from stem cell differentiation, to mammary gland and duct morphogenesis to motor neuron and axon guidance. ETV4 activity is regulated via the ERK/MAP signaling and a complex interplay of post-translation modifications such as sumoylation and ubiquitination. In cancers, ETV4 activates HIF and its subsequent proliferation genes and overexpression in prostate cells leads to oncogenesis. EMD-Millipore’s Anti-ETV4 mouse monoclonal antibody has been tested in western blot against K562 cell lysates as well as recombinantly expressed ETV4 protein.
      References
      Product Information
      FormatAscites
      Control
      • K562 cell lysate
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect ETS translocation variant 4 protein using this mouse monoclonal antibody, Anti-ETV4 Antibody & clone 1A2G3 validated for use in western blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPurified recombinant fragment of human ETV4 expressed in E. Coli.
      Clone1A2G3
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • ETV4
      • E1AF
      • PEA3
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~54 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected ETV4 in K562 cell lysate.

      Optimal working dilutions must be determined by end user.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC681 04053252920509