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DR1064 Anti-EXT2 Mouse mAb (3G6)

DR1064
  
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      Overview

      Replacement Information

      Key Spec Table

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      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~100 kDa EXT2 protein in human spleen.
      Catalogue NumberDR1064
      Brand Family Calbiochem®
      SynonymsAnti-Exostoses 2
      Application Data
      Detection of human EXT2 by immunoblotting. Sample: Whole tissue extract from human spleen. Primary antibody: Anti-EXT2 Mouse mAb (3G6) (Cat. No. DR1064) (1-5 µg/ml). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.17.
      Positive controlHuman spleen
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 216-315 of human EXT2, expressed as a GST fusion protein
      Clone3G6
      HostMouse
      IsotypeIgG₁
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1064 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision04-November-2010 RFH
      SynonymsAnti-Exostoses 2
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Application Data
      Detection of human EXT2 by immunoblotting. Sample: Whole tissue extract from human spleen. Primary antibody: Anti-EXT2 Mouse mAb (3G6) (Cat. No. DR1064) (1-5 µg/ml). Detection: chemiluminescence.
      DescriptionPurifued mouse monoclonal antibody. Recognizes the ~100 kDa EXT2 protein.
      BackgroundEXT2 is one of two glycosyltransferases involved in the chain elongation step of heparan sulfate biosynthesis. Mutations in this gene cause the type II form of multiple exostoses.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 216-315 of human EXT2, expressed as a GST fusion protein
      Clone3G6
      IsotypeIgG₁
      Specieshuman
      Positive controlHuman spleen
      FormLiquid
      FormulationIn PBS, pH 7.17.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review