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ABC60 Anti-Endostatin Antibody

Anti-Endostatin Antibody detects level of Endostatin & has been published & validated for use in WB, IH(P).

Anti-Endostatin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: Col18a1 UniProt Number: P39061
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ABC60
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABC60
      ReplacesAB1880
      DescriptionAnti-Endostatin Antibody
      Alternate Names
      • Collagen alpha-1(XVIII) chain
      • Endostatin
      Background InformationEndostatin is a proteolytic cleavage product from the C-terminus of Collagen XVIII, which is itself a proteoglycan/collagen found in blood vessel walls and in basement membranes. Endostatin is a compact globular fold-containing protein with a Zn2+ binding site near its amino terminus. Originally identified in conditioned media of murine hemangioendothelioma (EOMA) cells, endostatin is generated from the parent collagen XVIII molecule involving intermediate glycosylated forms and the proteolytic activity of proteases such as cathepsin L, metalloproteases, and elastase. Endostatin has a wide-spread tissue expression and has been shown to co-localize with fibulins, nidogen-2 in basement membranes, and elastic fibers of veins. Endostatin has also been shown to cause a G1 arrest in endothelial cells and to induce apoptosis.
      References
      Product Information
      FormatAffinity Purified
      Control
      • A431 cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Endostatin Antibody detects level of Endostatin & has been published & validated for use in WB, IH(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Endostatin in mouse lung tissue.
      Biological Information
      ImmunogenLinear peptide corresponding to the nonhelical region 11 of mouse Endostatin.
      EpitopeNonhelical Region 11 Domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Endostatin at the nonhelical region 11 domain.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Col18a1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Endostatin potently inhibits endothelial cell proliferation and angiogenesis. May inhibit angiogenesis by binding to the heparan sulfate proteoglycans involved in growth factor signaling.

      SUBCELLULAR LOCATION: Secreted › extracellular space › extracellular matrix (By similarity).

      TISSUE SPECIFICITY: Expressed in liver, kidney, lung, skeletal muscle and testis.

      PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

      SEQUENCE SIMILARITIES: Belongs to the multiplexin collagen family.

      Contains 1 FZ (frizzled) domain.

      Contains 1 TSP N-terminal (TSPN) domain.
      Molecular Weight~28 kDa observed.
      A ~28 kDa endostatin fragment has been observed in western blot as a result of MMP-7 cleavage of collagen XVIII (Chang, J., et al. (2003). Invest Ophthalmol Vis Sci. 44:832).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in A431 cell lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected Endostatin in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABC60 04053252640681

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Anti-Endostatin Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Endostatin Antibody Certificates of Analysis

      TitleLot Number
      Anti-Endostatin - 2118989 2118989
      Anti-Endostatin - 2161183 2161183
      Anti-Endostatin - 2266775 2266775
      Anti-Endostatin - 2329824 2329824
      Anti-Endostatin - 3759979 3759979
      Anti-Endostatin - 4201258 4201258
      Anti-Endostatin - NRG1953336 NRG1953336
      Anti-Endostatin -2737759 2737759
      Anti-Endostatin -2803238 2803238
      Anti-Endostatin Polyclonal Antibody 3005310
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