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DR1044 Anti-FEN1 Mouse mAb (1E2)

Anti-FEN1 Mouse mAb (1E2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Flap Structure-Specific Endonuclease 1

DR1044
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      M
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~40 kDa FEN1 protein in HeLa NE cells.
      Catalogue NumberDR1044
      Brand Family Calbiochem®
      SynonymsAnti-Flap Structure-Specific Endonuclease 1
      Application Data
      Detection of human FEN1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-FEN1 Mouse mAb (1E2) (Cat. No. DR1044) (1:500-1:1000). Detection: chemiluminescence.
      References
      Product Information
      FormLiquid
      FormulationUndiluted ascites.
      Positive controlHeLa NE cells
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:500-1000)
      Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-111 of human FEN1, expressed as a GST fusion protein
      ImmunogenHuman
      Clone1E2
      HostMouse
      IsotypeIgM
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1044 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-Flap Structure-Specific Endonuclease 1
      ApplicationImmunoblotting (1:500-1000)
      Application Data
      Detection of human FEN1 by immunoblotting. Sample: Whole cell lysate from HeLa NE cells. Primary antibody: Anti-FEN1 Mouse mAb (1E2) (Cat. No. DR1044) (1:500-1:1000). Detection: chemiluminescence.
      DescriptionMouse monoclonal antibody supplied as undiluted ascites. Recognizes the ~40 kDa FEN1 protein.
      BackgroundFEN1 protein removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication.
      HostMouse
      Immunogen speciesHuman
      ImmunogenA recombinant polypeptide corresponding to amino acids 1-111 of human FEN1, expressed as a GST fusion protein
      Clone1E2
      IsotypeIgM
      Specieshuman
      Positive controlHeLa NE cells
      FormLiquid
      FormulationUndiluted ascites.
      PreservativeNone
      CommentsVariables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review