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MABS460-I-100UG Anti-FGF23 Antibody, clone 278-2

Anti-FGF23, clone 278-2, Cat. No. MABS460-I, is a mouse monoclonal antibody that detects Fibroblast growth factor 23 and is tested for use in Flow Cytometry and Western Blotting.

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MABS460-I-100UG
100 μg  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABS460-I-100UG
      DescriptionAnti-FGF23 Antibody, clone 278-2
      Alternate Names
      • Fibroblast growth factor 23
      • FGF-23
      • Phosphatonin
      • Tumor-derived hypophosphatemia-inducing factor
      Background InformationFibroblast growth factor 23 (UniProt: Q9GZV9; also known as FGF-23, Phosphatonin, Tumor-derived hypophosphatemia-inducing factor) is encoded by the FGF23 (also known as HYPF, UNQ3027/PRO9828) gene (Gene ID: 8074) in human. FGF23 is a growth factor that is produced primarily in the bone tissue and its primary hormonal function is to regulate phosphate homeostasis and vitamin D metabolism. Unlike other FGFs, it has a low affinity for heparin sulfate, which allows it to enter the circulation and act as an endocrine hormone. It inhibits renal tubular phosphate transport by reducing sodium-dependent phosphate transport protein 2A (SLC34A1) levels. FGF23 binding to FGFR requires a co‐receptor, Klotho, which acts as a scaffold for docking with FGFR1. Together with Klotho, it modulates phosphate reabsorption and both 1 ‐hydroxylation and 24‐hydroxylation in the renal proximal tubules. FGF23 is reported to inhibit the expression of 1 ‐hydroxylase while increasing the expression of 24‐hydroxylase, thus reducing the concentration of active 1,25(OH)2D. It can also act directly on the parathyroid to reduce parathyroid hormone secretion. (Ref.: Almel, EA., et al. (2019). J. Bone Min. Res (JMBR Plus). 3(8): e10190).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-FGF23, clone 278-2, Cat. No. MABS460-I, is a mouse monoclonal antibody that detects Fibroblast growth factor 23 and is tested for use in Flow Cytometry and Western Blotting.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesTested Applications:\

      Flow Cytometry Analysis (FC): 1 µg of this antibody detected Fibroblast growth factor 23 (FGF23) in one million Jurkat cells pretreated with Brefeldin A (1 µg/mL 4 h).
      Biological Information
      ImmunogenRecombinant human Fibroblast growth factor 23 (FGF23).
      EpitopeUnknown
      Clone278-2
      Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 278.2 is a mouse monoclonal antibody that detects human Fibroblast growth factor 23 (FGF-23).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FGF23
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~37 kDa observed; 27.95 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Human brain tissue lysates.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected FGF23 in Human brain tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS460-I-100UG 04065266791419