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MABE227 Anti-FTO Antibody, clone 5-2H10

Anti-FTO Antibody, clone 5-2H10 detects level of FTO & has been published & validated for use in Western Blotting, IHC(P).

Anti-FTO Antibody, clone 5-2H10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: FTO UniProt Number: Q9C0B1
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MABE227
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE227
      DescriptionAnti-FTO Antibody, clone 5-2H10
      Alternate Names
      • Alpha-ketoglutarate-dependent dioxygenase FTO
      • Fat mass and obesity-associated protein
      Background InformationAlpha-ketoglutarate-dependent dioxygenase FTO (Fat mass and obesity-associated protein) is a ubiquitous nuclear protein which belongs to the FTO family. It is a dioxygenase enzyme that dealkylates nucleic acids by oxidative demethylation in the presence of oxygen, alpha-ketoglutarate and iron (Fe2+). FTO is highly active on single-stranded RNA molecules with 3-methyluracil residues and on single-stranded DNA containing 3-methylthymine residues, and has relatively low activity on single-stranded DNA containing either 3-methylcytosine or 3-methyladenine. There has been no observed activity on double-stranded DNA. FTO is highly abundant in the hypothalamus, pituitary, and adrenal glands. It plays an important role in energy homeostasis and the regulation of fat accumulation in the body, and may be involved in obesity. Mutated FTO proteins are linked to a syndrome characterized by retarded growth, delayed development, coarse facies and early death.
      References
      Product Information
      FormatPurified
      Control
      • Human kidney tissue lysate
      PresentationPurified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-FTO Antibody, clone 5-2H10 detects level of FTO & has been published & validated for use in Western Blotting, IHC(P).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected FTO in glandular cells of rat pituitary gland tissue.
      Biological Information
      ImmunogenRecombinant protein corresponding to human FTO.
      Clone5-2H10
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes.
      Gene Symbol
      • FTO
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: Dioxygenase that repairs alkylated DNA and RNA by oxidative demethylation. Has highest activity towards single-stranded RNA containing 3-methyluracil, followed by single-stranded DNA containing 3-methylthymine. Has low demethylase activity towards single-stranded DNA containing 1-methyladenine or 3-methylcytosine. Has no activity towards 1-methylguanine. Has no detectable activity towards double-stranded DNA. Requires molecular oxygen, alpha-ketoglutarate and iron. Contributes to the regulation of the global metabolic rate, energy expenditure and energy homeostasis. Contributes to the regulation of body size and body fat accumulation.

      COFACTOR: Binds 1 Fe2+ ion per subunit.

      ENZYME REGULATION: Activated by ascorbate. Inhibited by N-oxalylglycine, fumarate and succinate.

      SUBUNIT STRUCTURE: Monomer. May also exist as homodimer.

      SUBCELLULAR LOCATION: Nucleus.

      TISSUE SPECIFICITY: Ubiquitously expressed, with relatively high expression in adrenal glands and brain; especially in hypothalamus and pituitary.

      DOMAIN: The 3D-structure of the Fe2OG dioxygenase domain is similar to that of the Fe2OG dioxygenase domain found in the bacterial DNA repair dioxygenase alkB and its mammalian orthologs, but sequence similarity is very low. As a consequence, the domain is not detected by protein signature databases.
      Molecular Weight~58 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human kidney tissue lysate.

      Western Blot Analysis: 1 µg/mL of this antibody detected FTO in 10 µg of human kidney tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE227 04053252594915

      Documentation

      Anti-FTO Antibody, clone 5-2H10 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-FTO Antibody, clone 5-2H10 Certificates of Analysis

      TitleLot Number
      Anti-FTO, clone 5-2H10 - 2385976 2385976
      Anti-FTO, clone 5-2H10 - 2295485 2295485
      Anti-FTO, clone 5-2H10 - 3288501 3288501
      Anti-FTO, clone 5-2H10 - 3384549 3384549
      Anti-FTO, clone 5-2H10 - 3524847 3524847
      Anti-FTO, clone 5-2H10 - 3916197 3916197
      Anti-FTO, clone 5-2H10 - Q1984921 Q1984921
      Anti-FTO, clone 5-2H10 -2586547 2586547
      Anti-FTO, clone 5-2H10 -2801049 2801049
      Anti-FTO, clone 5-2H10 -2859159 2859159
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      Brochure

      Title
      New Products: Volume 3, 2012

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies