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PC69 Anti-Fas (Ab-1) (321-335) Rabbit pAb

Anti-Fas (Ab-1) (321-335), rabbit polyclonal, recognizes the ~44-46 kDa Fas in HL60 cells. It is validated for use in WB and IHC (frozen and paraffin sections).

Anti-Fas (Ab-1) (321-335) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-APO-1, Anti-CD95

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, Op, RRbPolyclonal Antibody
      Description
      OverviewRecognizes the ~44-46 kDa Fas protein in HL60 cells and color tissue.
      The immunogen, Fas Peptide (Cat. No. PP62), is available for competition studies.
      Catalogue NumberPC69
      Brand Family Calbiochem®
      SynonymsAnti-APO-1, Anti-CD95
      References
      ReferencesCheng, J., et al. 1995. J. Immunol. 154, 1239.
      Stanger, B.Z., et al. 1995. Cell 81, 513.
      Grell, M., et al. 1994. Eur. J. Immunol. 24, 2563.
      Owen-Schaub, L.B., et al. 1994. Cancer Res. 54, 1580.
      Itoh, N. and Nagata, S. 1993. J. Biol. Chem. 268, 10932.
      Oehm, A., et al. 1992. J. Biol. Chem. 267, 10709.
      Watanabe-Fukunaga, R., et al. 1992. J. Immunol. 148, 1274.
      Itoh, N., et al. 1991. Cell 66, 233.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Positive controlHL-60 or colon tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesFrozen Sections (2.5 µg/ml)
      Immunoblotting (2.5 µg/ml)
      Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
      Application CommentsWill detect Fas using frozen sections but not immunoblots of mouse and rat samples. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide (DSENSNFRNEIQSLV) corresponding to amino acids 321-335 of human Fas
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Opossum
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC69 0

      Documentation

      Anti-Fas (Ab-1) (321-335) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Fas (Ab-1) (321-335) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC69

      References

      Reference overview
      Cheng, J., et al. 1995. J. Immunol. 154, 1239.
      Stanger, B.Z., et al. 1995. Cell 81, 513.
      Grell, M., et al. 1994. Eur. J. Immunol. 24, 2563.
      Owen-Schaub, L.B., et al. 1994. Cancer Res. 54, 1580.
      Itoh, N. and Nagata, S. 1993. J. Biol. Chem. 268, 10932.
      Oehm, A., et al. 1992. J. Biol. Chem. 267, 10709.
      Watanabe-Fukunaga, R., et al. 1992. J. Immunol. 148, 1274.
      Itoh, N., et al. 1991. Cell 66, 233.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-September-2007 RFH
      SynonymsAnti-APO-1, Anti-CD95
      ApplicationFrozen Sections (2.5 µg/ml)
      Immunoblotting (2.5 µg/ml)
      Paraffin Sections (2.5 µg/ml, pressure cooker pre-treatment required)
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~44-46 kDa Fas protein.
      BackgroundFas/APO-1 was originally described as a cell surface antigen on activated human T and B lymphocytes and a variety of malignant human lymphoid cell lines. The gene encoding the human fas antigen consists of 9 exons, spans more than 26 kb of DNA and shares 58.5% homology with the mouse homolog. The protein contains a single transmembrane domain which is rich in cysteine residues and an inhibitory as well as a signal transducing or death domain in the cytoplasmic domain. Ligation or crosslinking of the extracellular domain by fas ligand or by antibody elicits a characteristic apoptotic response in susceptible cells including lymphoid cells and tumors as well as non-hematopoietic tumors. Upon activation, fas associates with a number of other proteins to mediate the response. Fas is a member of the TNF receptor family and shares some sequence homology with the TNF receptor but does not appear to physically interact with TNF-R itself.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide (DSENSNFRNEIQSLV) corresponding to amino acids 321-335 of human Fas
      IsotypeIgG
      Specieshuman, mouse, opossum, rat
      Positive controlHL-60 or colon tissue
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsWill detect Fas using frozen sections but not immunoblots of mouse and rat samples. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesCheng, J., et al. 1995. J. Immunol. 154, 1239.
      Stanger, B.Z., et al. 1995. Cell 81, 513.
      Grell, M., et al. 1994. Eur. J. Immunol. 24, 2563.
      Owen-Schaub, L.B., et al. 1994. Cancer Res. 54, 1580.
      Itoh, N. and Nagata, S. 1993. J. Biol. Chem. 268, 10932.
      Oehm, A., et al. 1992. J. Biol. Chem. 267, 10709.
      Watanabe-Fukunaga, R., et al. 1992. J. Immunol. 148, 1274.
      Itoh, N., et al. 1991. Cell 66, 233.