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MABE1154 Anti-Fibrillarin Antibody, clone 38F3

Anti-Fibrillarin, clone 38F3, Cat. No. MABE1154, is a highly specific mouse monoclonal antibody that targets Fibrillarin/Nop1p and has been tested in Immunocytochemistry and Western Blotting.

Anti-Fibrillarin Antibody, clone 38F3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: NOP1, LOT3, YDL014W, D2870 UniProt Number: P15646
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MABE1154
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, Yeast, Dr, R, HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1154
      DescriptionAnti-Fibrillarin Antibody, clone 38F3
      Alternate Names
      • Histone-glutamine methyltransferase
      • U3 small nucleolar RNA-associated protein NOP1
      • Nucleolar protein 1
      • U3 snoRNA-associated protein NOP1
      Background InformationrRNA 2'-O-methyltransferase fibrillarin (UniProt: P15646; also known as Histone-glutamine methyltransferase, U3 small nucleolar RNA-associated protein NOP1, Nucleolar protein 1, U3 snoRNA-associated protein NOP1) is encoded by the NOP1 (also known as LOT3, YDL014W, D2870) gene (Gene ID: 851548) in yeast. Fibrillarin is a nucleolar protein that is highly conserved throughout evolution. Human fibrillarin is 67% identical to its yeast homolog Nop1p. Fibrillarin is a protein component of C/D box small nucleolar ribonucleoproteins (snoRNPs) that direct 2 -O-methylation of rRNA and is also involved in other aspects of rRNA processing. Unlike snRNAs, which have a cytoplasmic phase of maturation, snoRNAs are restricted to the nucleus. Fibrillarin is essential for yeast viability and fibrillarin knockout is shown to be lethal in S. cerevisiae; however, expression of human or Xenopus fibrillarin overcome this lethality. The first 80 amino acids from the N terminal region of fibrillarin contain multiple RGG, or arginine-glycine-glycine (GAR). The remaining ~240 amino acids consist of the so called fibrillarin domain. NOP1 depleted strains of yeast are greatly impaired in the production of cytoplasmic ribosomes, and they have a reduced level of rRNA. Patients with scleroderma are reported to have strong circulating autoantibodies to fibrillarin. (Ref.: Tollervey, D et al. (1991). EMBO J. 10(3):573-83; Newton, K et al. (2003). Mol. Cell. Biol. 23(23): 8519-8527).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing PBS with 0.03% sodium azide with 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Fibrillarin, clone 38F3, Cat. No. MABE1154, is a highly specific mouse monoclonal antibody that targets Fibrillarin/Nop1p and has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A representative lot detected Fibrillarin in human SH-SY5Y cells (Courtesy of Dr Gerry Shaw).

      Immunocytochemistry Analysis: A representative lot detected Fibrillarin in Immunocytochemistry applications (Newton, K., et. al. (2003). Mol. Cell. Biol. 23(23):8519-27).
      Biological Information
      ImmunogenYeast nuclear extract
      Clone38F3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 38F3 specifically detects Fibrillarin/Nop1p in multiple mammalian species and in yeast.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Yeast
      • Drosophila
      • Rat
      • Human
      Species Reactivity NoteDrosophila, Human, Mouse, Rat, Yeast.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NOP1
      • LOT3
      • YDL014W
      • D2870
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~34 kDa observed; 34.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysates.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected Fibrillarin in 10 µg of HeLa cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1154 04054839178948