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MABF2660-100UL Anti-Flagellar Membrane Glycoprotein 1B (FMG-1B) Antibody, clone 8

Anti-Flagellar Membrane Glycoprotein 1B (FMG-1B), clone 8, Cat. No. MABF2660, is a mouse monoclonal antibody that detects FMG-1B and is tested for use in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blotting.

MABF2660-100UL
100 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberMABF2660-100UL
      DescriptionAnti-Flagellar Membrane Glycoprotein 1B (FMG-1B) Antibody, clone 8
      Alternate Names
      • FMG-1B
      Background InformationFlagella membrane glycoprotein 1B (UniProt: Q84X68; also known as FMG-1B) is derived from the flagella of single cell green algae Chlamydomonas reinhardtii. FMG-1B is a major membrane glycoprotein in the flagella of single green alga, Chlamydomonas. It is exposed at the flagellar surface and is reported to be the only flagellar protein in contact with the substrate during whole cell gliding motility and microsphere movement. It contains an N-terminal signal sequence, a transmembrane domain (aa 437-4160), and a short cytoplasmic tail of 17 amino acids that extends into the flagellar matrix. Chlamydomonas are reported to exhibit four types of flagella-dependent motilities described as: forward swimming where flagella leads the cell body, a reverse direction swim using a symmetrical sine wave pattern, flagellar surface interaction with substrate to facilitate whole cell gliding, and the intraflagellar transport (IFT) which moves multi-subunit protein complexes from the flagellar base to the tip in an antegrade direction. The anterograde IFT is reported to be powered by the activity of kinesin 2. Although FMG-1B is shown to be distributed along the surface of the flagellum, it also serves as a cargo for IFT machinery.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Applications
      ApplicationAnti-Flagellar Membrane Glycoprotein 1B (FMG-1B), clone 8, Cat. No. MABF2660, is a mouse monoclonal antibody that detects FMG-1B and is tested for use in ELISA, Immunofluorescence, Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunoprecipitation
      • ELISA
      Application NotesWestern Blotting Analysis: A representative lot detected Flagellar Membrane Glycoprotein 1B (FMG-1B) in Western Blotting applications (Dentler, W., et. al. (2013). PLoS One. 8(1):e53366; Bloodgood, R.A., et. al. (1986). J Cell Biol. 102(5):1797-812).

      ELISA Analysis: A representative lot detected Flagellar Membrane Glycoprotein 1B (FMG-1B) in ELISA applications (Bloodgood, R.A., et. al. (1986). J Cell Biol. 102(5):1797-812).

      Immunoprecipitation Analysis: A representative lot detected Flagellar Membrane Glycoprotein 1B (FMG-1B) in Immunoprecipitation applications (Bloodgood, R.A., et. al. (1994). J Cell Biol. 127(3):803-11).

      Immunofluorescence Analysis: A representative lot detected Flagellar Membrane Glycoprotein 1B (FMG-1B) in Immunofluorescence applications (Kumar, D., et. al. (2016). J Cell Sci. 129(5):943-56; Bloodgood, R.A., et. al. (2019). J Cell Sci. 132(16):jcs233429).

      Immunofluorescence Analysis: A 1:250 dilution from a representative lot detected Flagellar Membrane Glycoprotein 1B (FMG-1B) in gluteraldehyde fixed Chlamydomonas reinhardtii.

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenPurified flagellar protein from Chlamydomonas reinhardtii.
      EpitopeUnknown
      Clone8
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 8 is a mouse monoclonal antibody that detects Flagellar Membrane Glycoprotein 1B (FMG-1B) in Chlamydomonas reinhardtii.
      IsotypeIgG1κ
      Species Reactivity
      • A Broad Range Of Species
      Species Reactivity NoteChlamydomonas reinhardtii (Green Algae).
      Antibody TypeMonoclonal Antibody
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight412.65 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -10°C to -25°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2660-100UL 04065265324144