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ABS2200-25UL Anti-FtsZ (GTPase)

Anti-FtsZ (GTPase), Cat. No. ABS2200, is a rabbit polyclonal antibody that detects Cell division protein FtsZ and has been tested for use in Immunofluorescence and Western Blotting.

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ABS2200-25UL
25 µL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberABS2200-25UL
      DescriptionAnti-FtsZ (GTPase)
      Alternate Names
      • Cell division protein FtsZ
      • Cell Division FtsZ GTPase
      Background InformationCell division protein FtsZ (UniProt: P17865; also known as Cell Division FtsZ GTPase, FtsZ) is encoded by the FtsZ (also known as BSU15290) gene (Gene ID: 935971) in Bacillus subtilis. FtsZ is homodimeric prokaryotic homologue of tubulin with GTPase activity. It is found in nearly all bacteria. It polymerizes in the presence of GTP to form higher-ordered polymers. It contains three GTP-binding sites that are located in the N-terminal region. The less conserved C-terminal domain contains residues that are important for GTP hydrolysis. FtsZ is shown to be essential for bacterial cell division and in association with FtzA (FtsAZ) it forms a contractile ring structure (Z ring) at the site of cell division. The regulation of the ring assembly controls the timing and the location of cell division. One of the major functions of FtsZ is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. The FtsZ treadmilling rate controls the rate of peptidoglycan synthesis and cell division. During bacterial cell division FtsZ co-localizes with SpoIIE, a dual function protein that contributes to the switch form medial to polar cell division during sporulation. The anti-FtsZ blot has also been used as control for sample loading.
      (Ref.: Bisson-Filho, AW., et al., (2017). Science 355(6326); 739-743; Feucht, A., and Jeffery Errington, J (2005). Microbiology 151(6); 2053-2064; Lucet, I., et al. (2000). EMBO J. 19(7); 1467-1475). Lytvynenko, I., et al. (2019). Cell. 178(1); 76-90)
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
      Applications
      ApplicationAnti-FtsZ (GTPase), Cat. No. ABS2200, is a rabbit polyclonal antibody that detects Cell division protein FtsZ and has been tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected FtsZ (GTPase) in Western Blotting applications (Lucet, I., et. al. (2000). EMBO J. 19(7):1467-75; Bisson-Filho, A.W., et. al. (2017). Science. 355(6326):739-743).

      Immunofluorescence Analysis: A representative lot detected FtsZ (GTPase) in Immunofluorescence applications (Daniel, R.A., et. al. (2000). Mol Microbiol. 35(2):299-311).
      Biological Information
      ImmunogenFull length purified FtsZ from Bacillus subtilis.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Cell Division Protein FTsZ in Bacillus subtilis.
      IsotypeIgG
      Species Reactivity
      • Bacteria
      Species Reactivity NoteBacillus subtilis.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FtsZ
      • BSU15290
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~44 kDa observed; 40.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in GFP-FtsZ & TrsZ in Bacillus subtilis 168ca wild type.

      Western Blotting Analysis: A 1:5,000 dilution of this antibody detected GFP-FtsZ and FtsZ in wild type Bacillus subtilis 168ca.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS2200-25UL 04054839638510