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MABN2396 Anti-GCAP1 Antibody, clone 6B12

Anti-GCAP1, clone 6B12, Cat. No. MABN2396, is a highly specific mouse monoclonal antibody that targets Guanylyl cyclase-activating protein 1 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-GCAP1 Antibody, clone 6B12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Guca1a, Gcap, Gcap1, Guca1 UniProt Number: P43081
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MABN2396
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, H, M, B, PorcineIF, IH(P), IP, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN2396
      DescriptionAnti-GCAP1 Antibody, clone 6B12
      Alternate Names
      • Guanylyl cyclase-activating protein 1
      • Guanylate cyclase activator 1A
      Background InformationGuanylyl cyclase-activating protein 1 (UniProt: P43081; also known as GCAP 1, Guanylate cyclase activator 1A) is encoded by the Guca1a (also known as Gcap, Gcap1, Guca1) gene (Gene ID: 14913) in murine species. GCAP1 is a membrane bound protein that stimulates retinal guanylyl cyclase when free calcium ions concentration is low and inhibits guanylyl cyclase when free calcium ions concentration is elevated. This Ca2+-sensitive regulation of retinal guanylyl cyclase appears to be a key event in recovery of the dark state of rod photoreceptors following light exposure. GCAP1 is present in rod and cone photoreceptor outer segments where phototransduction occurs. It binds three calcium ions (via EF-hands 2, 3 and 4) when calcium levels are high. Binds Mg2+ when calcium levels are low. Three calcium-binding sites are identified (aa 64-75; 100-111; and 144-155). GCAP1 restores the calcium sensitivity of guanylyl cyclase in a reconstituted system, and it decreases the sensitivity, time-to-peak, and recovery time of the light response following its introduction into intact rod outer segments. (Ref.: Gorczyca WA (1995). J. Biol Chem. 270(37); 22029-36; Schrem A et al. (1999). J Biol Chem. 274(10); 6244-49).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GCAP1, clone 6B12, Cat. No. MABN2396, is a highly specific mouse monoclonal antibody that targets Guanylyl cyclase-activating protein 1 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunofluorescence Analysis: A representative lot detected GCAP1 in Immunofluorescence applications (Zulliger, R., et. al. (2015). J Biol Chem. 290(6):3488-99).

      Immunofluorescence Analysis: A 1:10 dilution from a representative lot detected GCAP1 in mouse retina.

      Western Blotting Analysis: A representative lot detected GCAP1 in Western Blotting applications (Zulliger, R., et. al. (2015). J Biol Chem. 290(6):3488-99).

      Immunoprecipitation Analysis: A representative lot detected GCAP1 in Immunoprecipitation applications (Zulliger, R., et. al. (2015). J Biol Chem. 290(6):3488-99).

      Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected GCAP1 in human retina tissue.
      Biological Information
      ImmunogenGST-fusion protein containing 45 amino acids from the C-terminal region of murine GCAP1. It does not display any homology with GCAP2.
      EpitopeC-terminus
      Clone6B12
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6B12 detects Guanylyl cyclase-activating protein 1 in Bovine, Human, Mouse, Porcine, and Rat. It targets an epitope within 45 amino acids from the C-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Rat
      • Human
      • Mouse
      • Bovine
      • Porcine
      Species Reactivity NoteBovine, Human, Mouse, Porcine, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Guca1a
      • Gcap
      • Gcap1
      • Guca1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight23 kDa observed; 22.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in C57BL/6 mouse retinal tissue lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected GCAP1 in C57BL/6 mouse retinal tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN2396 04054839367175