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DR1045 Anti-GCLC Mouse mAb (3H1)

Anti-GCLC, mouse monoclonal, clone 3H1, recognizes the ~70 kDa GCLC protein in HeLa and A431 cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.

Anti-GCLC Mouse mAb (3H1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Glutamate-Cysteine Ligase Catalytic Subunit

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DR1045
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~70 kDa GCLC protein in HeLa and A431 cells.
      Catalogue NumberDR1045
      Brand Family Calbiochem®
      SynonymsAnti-Glutamate-Cysteine Ligase Catalytic Subunit
      Application Data
      Detection of human GCLC by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-GCLC Mouse mAb (3H1) (Cat. No. DR1045) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GCLC by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GCLC Mouse mAb (3H1) (Cat. No. DR1045) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Negative control293T cells
      Positive controlA431 cells, HeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids 528-638 of human GCLC, expressed as a GST fusion protein
      Clone3H1
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1045 0

      Documentation

      Anti-GCLC Mouse mAb (3H1) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-Glutamate-Cysteine Ligase Catalytic Subunit
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human GCLC by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-GCLC Mouse mAb (3H1) (Cat. No. DR1045) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GCLC by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-GCLC Mouse mAb (3H1) (Cat. No. DR1045) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monolconal antibody. Recognizes the ~70 kDa GCLC protein.
      BackgroundGlutamate-cysteine ligase, also known as γ-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Deficiency of γ-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids 528-638 of human GCLC, expressed as a GST fusion protein
      Clone3H1
      IsotypeIgG₁
      Specieshuman
      Positive controlA431 cells, HeLa cells
      Negative control293T cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review