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MABN1556-25UG Anti-GGA3 Antibody, clone 3G5.1

Anti-GGA3, clone 3G5.1, Cat. No. MABN1556, is a highly specific mouse monoclonal antibody that targets ADP-ribosylation factor-binding protein GGA3 and has been tested for use in Western Blotting.

Research Category: Neuroscience Gene Symbol: GGA3, KIAA0154 UniProt Number: Q9NZ52
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MABN1556-25UG
25 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1556-25UG
      DescriptionAnti-GGA3 Antibody, clone 3G5.1
      Alternate Names
      • ADP-ribosylation factor-binding protein GGA3
      • Golgi-localized gamma ear-containing ARF-binding protein 3
      Background InformationADP-ribosylation factor-binding protein GGA3 (UniProt: Q9NZ52; also known as Golgi-localized, gamma ear-containing, ARF-binding protein 3) is encoded by the GGA3 (also known as KIAA0154) gene (Gene ID: 23163) in human. GGA3 is a monomeric, ubiquitously expressed protein found in trans-Golgi network (TGN) membrane and endosome membrane. It plays a vital role in protein sorting and trafficking between the trans-Golgi network and endosomes. It mediates the ARF-dependent recruitment of clathrin to the TGN and binds ubiquitinated proteins and membrane cargo molecules with a cytosolic acidic cluster-dileucine (AC-LL) motif. Four isoforms of GGA3 have been described that are produced by alternative splicing. It contains a VHS domain (aa 16-146) that functions as a recognition module for sorting signals composed of an acidic cluster followed by two leucines. Its GAT domain (aa 171-298) is responsible for interaction with ARF-GTP, UBC, and RAB GTPase Binding Effector Protein 1 (RABEP1) and prevents ARF-GTP hydrolysis. GGA3 also has a GAE domain (aa 594-715) that binds accessory proteins regulating GGAs function. GGA3 also contains an auto-inhibitory motif (aa 391-395). GGA3 can be phosphorylated by casein kinase 2 and dephosphorylated by protein phosphatase 2A. GGA3 phosphorylation allows the internal AC-LL motif to bind VHS domain and inhibit the recognition of cargo signals.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-GGA3, clone 3G5.1, Cat. No. MABN1556, is a highly specific mouse monoclonal antibody that targets ADP-ribosylation factor-binding protein GGA3 and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST/His-tagged recombinant fragment corresponding to 103 amino acids from the internal region of human ADP-ribosylation factor-binding protein GGA3.
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3G5.1 detects ADP-ribosylation factor-binding protein GGA3 in human and murine cells. It targets an epitope within 103 amino acids from the internal region.
      IsotypeIgG2b
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • GGA3
      • KIAA0154
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~80 kDa observed; 78.32 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Neuro-2a cell lysate.
      Western Blotting Analysis: 1 µg/mL of this antibody detected GGA3 in 10 µg of Neuro-2a cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1556-25UG 04054839494215

      Documentation