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CB1042 Anti-GSN Mouse mAb (3G5)

Anti-GSN, mouse monoclonal, clone 3G5, recognizes the ~75 kDa GSN protein in HeLa cells and human tonsil tissue. It is validated for use in ELISA, WB, and IHC (paraffin sections).

Anti-GSN Mouse mAb (3G5) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Gelsolin

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CB1042
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~75 kDa GSN protein in HeLa cells and human tonsil tissue.
      Catalogue NumberCB1042
      Brand Family Calbiochem®
      SynonymsAnti-Gelsolin
      Application Data
      Detection of human GSN by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-GSN Mouse mAb (3G5) (Cat. No. CB1042) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GSN by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human tonsil tissue. Primary antibody: Anti-GSN Mouse mAb (3G5) (Cat. No. CB1042) (1 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHeLa cells, Human tonsil tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (1 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corresponding to amino acids of 673-783 human GSN, expressed as a GST fusion protein.
      Clone3G5
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CB1042 0

      Documentation

      Anti-GSN Mouse mAb (3G5) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-November-2010 RFH
      SynonymsAnti-Gelsolin
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (1 µg/ml)
      Application Data
      Detection of human GSN by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-GSN Mouse mAb (3G5) (Cat. No. CB1042) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human GSN by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human tonsil tissue. Primary antibody: Anti-GSN Mouse mAb (3G5) (Cat. No. CB1042) (1 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~75 kDa GSN protein.
      BackgroundThe protein encoded by this gene binds to the "plus" ends of actin monomers and filaments to prevent monomer exchange. The encoded calcium-regulated protein functions in both assembly and disassembly of actin filaments.
      HostMouse
      ImmunogenA recombinant polypeptide corresponding to amino acids of 673-783 human GSN, expressed as a GST fusion protein.
      Clone3G5
      IsotypeIgG1
      Specieshuman
      Positive controlHeLa cells, Human tonsil tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review