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MABF2069 Anti-Glucuronoxylomannan (GXM) Antibody, clone 18B7

Anti-Glucuronoxylomannan (GXM), clone 18B7, Cat. No. MABF2069, is a mouse monoclonal antibody for detection of Cryptococcus neoformans Glucuronoxylomannan and has been tested for use in and ELISA, Immunodepletion, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry (Paraffin),

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: N/A
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MABF2069
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MELISA, Immunodepletion, ICC, IF, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF2069
      DescriptionAnti-Glucuronoxylomannan (GXM) Antibody, clone 18B7
      Alternate Names
      • GXM
      Background InformationCryptococcus neoformans is an encapsulated yeast-like fungus that is reported to causes systemic infections, including fatal meningoencephalitis. Glucuronoxylomannan (GXM) is the principal constituent of the Cryptococcus neoformans capsule that modulates the inflammatory response of human monocytes in vitro. The basic structural unit of GXM is a tri-mannose repeat with a glucuronic acid residue in the first mannose. Its structure is shown to be further modified in individual strains by the addition of xylose substitutions on the mannan backbone. Six triads known as M1-M2 are shown to be present in various proportions in GXM from the various serotypes and M2 is reported to be the most common triad in serotype A GXM. Soluble GXM serves as an important virulence factor and contributes to the pathogenesis of infection, and high levels of GXM have been found in serum of patients with cryptococcosis. Clone 18B7 displays significant activity against GXM and is reported act by possibly hydrolyzing the oligosaccharide substrate. This antibody acts as a potent opsonin for Cryptococcus neoforman by increasing its phagocytosis. This antibody may also reduce GXM levels by interfering with the release of polysaccharide from the capsule. Administration of this antibody to mice is shown to rapidly clear serum Cryptococcal antigen. (Ref.: Bowen, A., et al. (2017). J. Biol. Chem. 292(2); 417-434; Martinez, LR., et al. (2004). Infect. Immun. 72(6); 3674 3679; Tissi, L., et al. (2004). Infect. Immun. 72(11); 6367-6372; Devi, SJN et al. (1991). Infect. Immun. 59(10); 3700-3707).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in PBS without azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Glucuronoxylomannan (GXM), clone 18B7, Cat. No. MABF2069, is a mouse monoclonal antibody for detection of Cryptococcus neoformans Glucuronoxylomannan and has been tested for use in and ELISA, Immunodepletion, Immunocytochemistry, Immunofluorescence, and Immunohistochemistry (Paraffin),
      Key Applications
      • ELISA
      • Immunodepletion
      • Immunocytochemistry
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A representative lot detected Glucuronoxylomannan (GXM) in Immunohistochemistry applications (Lendvai, N., et. al. (1999). J Infect Dis. 180(3):791-801; Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46).

      Immunodepletion Analysis: A representative lot immunodepleted Glucuronoxylomannan (GXM) in Immunodepletion applications (Lendvai, N., et. al. (1999). J Infect Dis. 180(3):791-801; Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Martinez, L.R., et. al. (2004). Infect Immun. 72(6):3674-9; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).

      Immunofluorescence Analysis: A representative lot detected Glucuronoxylomannan (GXM) in Immunofluorescence applications (Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).

      Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Glucuronoxylomannan (GXM) in heat killed cryptococcus cells.

      Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Glucuronoxylomannan (GXM) in S. neoformans-infected mouse spleen, mouse lung, mouse liver, and mouse brain tissues.

      ELISA Analysis: A representative lot detected Glucuronoxylomannan (GXM) in ELISA applications (Casadevall, A., et. al. (1998). Antimicrob Agents Chemother. 42(6):1437-46; Martinez, L.R., et. al. (2004). Infect Immun. 72(6):3674-9; Bowen, A., et. al. (2017). J Biol Chem. 292(2):417-434).
      Biological Information
      ImmunogenCryptococcus neoformans serotype A glucuronoxylomannan (GXM)-Tetanus Toxoid conjugate vaccine.
      Clone18B7
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 18B7 is a mouse monoclonal antibody that binds to Glucuronoxylomannan (GXM) from Cryptococcus neoformans.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse.
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • N/A
      Purification MethodProtein G purified
      Molecular WeightN/A
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

      Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF2069 04054839415258

      Documentation

      Anti-Glucuronoxylomannan (GXM) Antibody, clone 18B7 Certificates of Analysis

      TitleLot Number
      Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3160112 3160112
      Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3302958 3302958
      Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3506491 3506491
      Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3717006 3717006
      Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3735796 3735796
      Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3789393 3789393
      Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3800108 3800108
      Anti-Glucuronoxylomannan (GXM), clone 18B7 - 3929943 3929943
      Anti-Glucuronoxylomannan (GXM), clone 18B7 - 4001929 4001929
      Anti-Glucuronoxylomannan (GXM), clone 18B7 - 4013866 4013866
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