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PC53 Anti-Glutathione S-Transferase (Ab-1) Rabbit pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-GST

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PC53
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      S. japonicumRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes glutathione S-transferase. Good negative control for rabbit polyclonal antibodies.
      Catalogue NumberPC53
      Brand Family Calbiochem®
      SynonymsAnti-GST
      References
      ReferencesSmith, D.B. and Johnson, K.S. 1988. Gene 67, 31.
      Beekman, J.M., et al. 1994. Gene 146, 285.
      Poon, R.Y. and Hunt, T. 1994. Anal. Biochem. 218, 26.
      Fikrig, E., et al. 1990. Science 250, 553.
      Toye, B., et al. 1990. Infect. Immunol. 58, 3909.
      Product Information
      FormLiquid
      FormulationIn PBS, 0.2% Tween®-20 detergent.
      Positive controlRecombinant GST protein
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Affinity Chromatography
      Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Application NotesELISA (75/ng/well, capture antibody)
      Affinity Chromatography (see comments)
      Immunoblotting (0.5-1 µg/ml)
      Immunoprecipitation (10 µg/ml)
      Application CommentsDoes not cross-react with either human or E. coli derived glutathione-S-transferase. Can be used for affinity chromatography purification of GST fusion proteins. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant glutathione S-transferase from S. japonicum
      ImmunogenSchistosoma japonicum
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • S. japonicum
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC53 0

      Documentation

      Anti-Glutathione S-Transferase (Ab-1) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      References

      Reference overview
      Smith, D.B. and Johnson, K.S. 1988. Gene 67, 31.
      Beekman, J.M., et al. 1994. Gene 146, 285.
      Poon, R.Y. and Hunt, T. 1994. Anal. Biochem. 218, 26.
      Fikrig, E., et al. 1990. Science 250, 553.
      Toye, B., et al. 1990. Infect. Immunol. 58, 3909.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision26-February-2008 JSW
      SynonymsAnti-GST
      ApplicationELISA (75/ng/well, capture antibody)
      Affinity Chromatography (see comments)
      Immunoblotting (0.5-1 µg/ml)
      Immunoprecipitation (10 µg/ml)
      DescriptionPurified rabbit polyclonal antibody. Recognizes glutathione S-transferase
      BackgroundGlutathione S-transferase (GST) is commonly used as an epitope tag in generating fusion proteins. In these systems a ~26 kDa GST domain isolated from S. japonicum is cloned in-frame to the gene encoding the protein of interest in the appropriate expression vector system. The resulting fusion protein can then be expressed by either E. coli (pGEX expression vector system) or by yeast (pYEX™ expression vector system) in a soluble form. The resulting fusion proteins may then be further purified by immunoprecipitation or affinity chromatography with GST (Ab-1) or directly analyzed by western blotting with the antibody.
      HostRabbit
      Immunogen speciesSchistosoma japonicum
      Immunogenrecombinant glutathione S-transferase from S. japonicum
      IsotypeIgG
      Speciesnot E. coli, S. japonicum, not human
      Positive controlRecombinant GST protein
      FormLiquid
      FormulationIn PBS, 0.2% Tween®-20 detergent.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsDoes not cross-react with either human or E. coli derived glutathione-S-transferase. Can be used for affinity chromatography purification of GST fusion proteins. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesSmith, D.B. and Johnson, K.S. 1988. Gene 67, 31.
      Beekman, J.M., et al. 1994. Gene 146, 285.
      Poon, R.Y. and Hunt, T. 1994. Anal. Biochem. 218, 26.
      Fikrig, E., et al. 1990. Science 250, 553.
      Toye, B., et al. 1990. Infect. Immunol. 58, 3909.