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ABT65 Anti-HAX-1 Antibody

Anti-HAX-1 Antibody is an antibody against HAX-1 for use in WB & IC.

Anti-HAX-1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional, Cytoskeleton Gene Symbol: HAX1, HS1BP1 UniProt Number: O00165
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ABT65
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABT65
      DescriptionAnti-HAX-1 Antibody
      Alternate Names
      • HCLS1-associated protein X-1
      • HS1-associating protein X-1
      • HAX-1
      • HS1-binding protein 1
      • HSP1BP-1
      Background InformationHAX-1 (HS1-associated protein X-1) is a mitochondrial anti-apoptotic protein and is thought to be regulated by Omi/HtrA2. HAX-1 has been found to associate with BSEP, MDR1 and MDR2 ABC-transporters and interacts with cortactin to play a role in BSEP internalization from the apical membrane. HAX-1 and alphavbeta6 are found to be highly expressed in certain oral cancers and work together to enhance tumor invasion and progression. High expression of HAX-1 in many other cancer tissues shows that HAX-1 is important in neoplastic transformation.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Jurkat cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-HAX-1 Antibody is an antibody against HAX-1 for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blot Analysis: 0.1 µg/mL from a representative lot detected HAX-1 in 10 µg of HeLa cell lysate and rat liver tissue lysate.

      Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected HAX-1 in NIH/3T3 and HeLa cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the domain of human HAX-1 that is involved in HCLS1-, CASP9-, and GNA13-bindings.
      EpitopeHCLS1-, CASP9-, and GNA13-binding domains
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes HAX-1 at the domains involved in HCLS1-, CASP9-, and GNA13-binding.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is known to associate with hematopoietic cell-specific Lyn substrate 1, a substrate of Src family tyrosine kinases. It also interacts with the product of the polycystic kidney disease 2 gene, mutations in which are associated with autosomal-dominant polycystic kidney disease, and with the F-actin-binding protein, cortactin. It was earlier thought that this gene product is mainly localized in the mitochondria, however, recent studies indicate it to be localized in the cell body. Mutations in this gene result in autosomal recessive severe congenital neutropenia, also known as Kostmann disease. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].
      Gene Symbol
      • HAX1
      • HS1BP1
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Promotes cell survival. Potentiates GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. May regulate intracellular calcium pools.

      SUBUNIT STRUCTURE: Interacts with ABCB1, ABCB4 and ABCB11 By similarity. Directly associates with HCLS1/HS1, through binding to its N-terminal region. Interacts with CTTN. Interacts with PKD2. Interacts with GNA13. Interacts with CASP9. Interacts with ITGB6. Interacts with PLN and ATP2A2; these interactions are inhibited by calcium.

      SUBCELLULAR LOCATION: Mitochondrion. Endoplasmic reticulum. Nucleus membrane. Cytoplasmic vesicle (By similarity). Sarcoplasmic reticulum (By similarity).

      TISSUE SPECIFICITY: Ubiquitous. Up-regulated in oral cancers.

      PTM: Proteolytically cleaved by caspase-3 during apoptosis.

      INVOLVEMENT IN DISEASE: Defects in HAX1 are the cause of neutropenia severe congenital autosomal recessive type 3 (SCN3) [MIM:610738]; also known as Kostmann disease. A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 109/l and early onset of severe bacterial infections. Some patients affected by severe congenital neutropenia type 3 have neurological manifestations such as psychomotor retardation and seizures. Note=The clinical phenotype due to HAX1 deficiency appears to depend on the localization of the mutations and their influence on the transcript variants. Mutations affecting exclusively isoform 1 are associated with isolated congenital neutropenia, whereas mutations affecting both isoform 1 and isoform 5 are associated with additional neurologic symptoms. Ref.14 Ref.15 Ref.19 Ref.20

      SEQUENCE SIMILARITIES: Belongs to the HAX1 family.
      Molecular Weight~35 kDa observed.
      HAX-1 has been shown to migrate as a ~34-36 kDa band in WB (Ortiz, D.F., et al. (2004). JBC. 279(31):32761-32770).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Jurkat cell lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected HAX-1 in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABT65 04053252423413