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AB5378-50UL Anti-HCN2 Antibody

AB5378-50UL
50 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RIHC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5378-50UL
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-HCN2 Antibody
      Alternate Names
      • Hyperpolarization-Activated Cyclic Nucleotide-Gated Potassium Channel 2
      • BCNG2
      • HAC-1
      References
      Product Information
      FormatAffinity Purified
      Control
      • CONTROL ANTIGEN:

        Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile deionized water. For negative control, preincubate 1 μg of peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
      PresentationAffinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.05% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min).
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-HCN2 Antibody is validated for use in IH, WB for the detection of HCN2.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesWestern blot: 1:200-1:1,000 using ECL on rat brain membranes.

      Immunohistochemistry on rat brain sections.

      Dilutions should be made using a carrier protein such as BSA (1-3%)

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenPurified peptide from human HCN2 (amino acids 147-161) (Accession CAB42602).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityRecognizes HCN2 protein. The epitope is specific for HCN2 and is not present in any other known protein.
      Species Reactivity
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HCN2
      • BCNG-2
      • HAC-1
      • BCNG2
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q9UL51 # Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Produces a large instantaneous current. Activated by cAMP. Modulated by intracellular chloride ions and pH; acidic pH shifts the activation to more negative voltages (By similarity).
      SIZE: 889 amino acids; 96950 Da
      SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN1. Interacts with KCNE2 (By similarity).
      SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
      TISSUE SPECIFICITY: Highly expressed throughout the brain. Detected at low levels in heart.
      DOMAIN:SwissProt: Q9UL51 The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
      SIMILARITY: Belongs to the potassium channel HCN family. & Contains 1 cyclic nucleotide-binding domain.
      MISCELLANEOUS: Inhibited by extracellular cesium ions.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain lyophilized material at -20°C for up to 12 months. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size50 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-HCN2 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-HCN2 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-HCN2 - 2555338 2555338
      RABBIT ANTI-HCN2 -2514890 2514890
      RABBIT ANTI-HCN2 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2937877
      RABBIT ANTI-HCN2 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2925262
      RABBIT ANTI-HCN2 AFFINITY PURIFIED POLYCLONAL ANTIBODY 3058477
      RABBIT ANTI-HCN2 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2876895
      RABBIT ANTI-HCN2 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2227103 2227103

      References

      Reference overviewPub Med ID
      Regulation of the hyperpolarization-activated cationic current Ih in mouse hippocampal pyramidal neurones by vitronectin, a component of extracellular matrix
      Vasilyev, Dmitry V and Barish, Michael E
      J Physiol (Lond), 560:659-75 (2004)  2004

      15319414 15319414

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies