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07-2174 Anti-Heat Shock Protein 90α Antibody

Detect the Heat Shock Protein 90α protein using this Anti-Heat Shock Protein 90α validated for use in WB, IP, ICC & IHC.

Anti-Heat Shock Protein 90α Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Protein Trafficking Research Sub-Category: Chaperones Gene Symbol: HSP90AA1, HSPCAL4, HSPN, HSP86, Hsp89, HSP90N, HSPC1, HSP90A, Hsp90, HSPCAL1, HSPCA, LAP2 UniProt Number: P07900
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07-2174
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, CaWB, IP, ICC, IHCRbPurifiedPolyclonal Antibody
      Description
      Catalogue Number07-2174
      DescriptionAnti-Heat Shock Protein 90α Antibody
      Alternate Names
      • heat shock protein 90kDa alpha (cytosolic), class A member 1
      • Renal carcinoma antigen NY-REN-38
      • heat shock 90kD protein 1, alpha
      • heat shock 90kD protein, alpha-like 4
      • heat shock 90kDa protein 1, alpha
      • heat shock protein HSP 90-alpha
      • Heat shock 86 kDa
      • heat shock 90kD protein 1, alpha-like 4
      Background InformationThe Heat Shock Protein 90 family is known as a group of molecular chaperones involved in the regulation of maturation, degradation, and folding transport for a select, yet varied group of proteins primarily comprised of signaling molecules, but also including tyrosine kinases, G-protein subunits, transcription factors, and telomerase. Heat Shock Protein 90 has two isoforms that share a homology of 76%; Heat Shock Protein 90α (HSP90α) and Heat Shock Protein 90β (HSP90β). HSP90α contains a charged sequence linker, a C-terminal domain, and N-terminal domain and a middle domain. It is induced by oxidative stress and has been observed in exosomes where it is secreted into the extracellular environment following translocation via TGFα stimulus. Extracellular HSP90α functions to facilitate the migration of both dermal and epidermal cells through its interaction with cell surface receptors CD91 and LRP1. HSP90α interaction with TERT is a necessary component of TERT holoenzyme complex assembly and stabilization. It has also been shown to interact with a variety of other proteins such as FNIP1, HSF1, TOM34, AHSA1, SMYD3, and DNAJC7.
      References
      Product Information
      FormatPurified
      Control
      • HeLa cell lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect the Heat Shock Protein 90α protein using this Anti-Heat Shock Protein 90α validated for use in WB, IP, ICC & IHC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesWestern Blot (Snap i.d.®) Analysis: 1 µg/mL from a previous lot detected Heat Shock Protein 90α on 10 µg of NIH/3T3 cell lysate.

      Immunohistochemistry Analysis: 1:100 dilution from a previous lot detected Heat Shock Protein 90α in human prostate adenocarcinoma tissue.

      Immunocytochemistry Analysis: 1:500 dilution from a previous ot detected Heat Shock Protein 90α in A431 cells.

      Immunoprecipitation Analysis: 10 µg from a previous lot immunoprecipitated Heat Shock Protein 90A from HeLa cell lysate.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminus of human Heat Shock Protein 90α.
      EpitopeN-terminus
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes Heat Shock Protein 90α at the N-terminus.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Canine
      Species Reactivity NoteDemonstrated to react with human, mouse, and rat. Predicted to react with canine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryHSP90 proteins are highly conserved molecular chaperones that have key roles in signal transduction, protein folding, protein degradation, and morphologic evolution. HSP90 proteins normally associate with other cochaperones and play important roles in folding newly synthesized proteins or stabilizing and refolding denatured proteins after stress. There are 2 major cytosolic HSP90 proteins, HSP90AA1, an inducible form, and HSP90AB1 (MIM 140572), a constitutive form. Other HSP90 proteins are found in endoplasmic reticulum (HSP90B1; MIM 191175) and mitochondria (TRAP1; MIM 606219) (Chen et al., 2005 [PubMed 16269234]).[supplied by OMIM].
      Gene Symbol
      • HSP90AA1
      • HSPCAL4
      • HSPN
      • HSP86
      • Hsp89
      • HSP90N
      • HSPC1
      • HSP90A
      • Hsp90
      • HSPCAL1
      • HSPCA
      • LAP2
      Purification MethodProtein A
      UniProt Number
      UniProt SummaryFUNCTION: Molecular chaperone. Has ATPase activity (By similarity).

      SIZE: 732 amino acids; 84660 Da

      SUBUNIT: Homodimer. Interacts with AHSA1, SMYD3 and TOM34. Interacts with FNIP1.

      SUBCELLULAR LOCATION: Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

      SIMILARITY: Belongs to the heat shock protein 90 family.
      Molecular Weight~ 90 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa cell lysate.

      Western Blot Analysis: 0.0625 µg/mL of this antibody detected Heat Shock Protein 90α on 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-2174 04053252375446

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Anti-Heat Shock Protein 90α Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Heat Shock Protein 90α Antibody Certificates of Analysis

      TitleLot Number
      Anti-Heat Shock Protein 90 - 2073042 2073042
      Anti-Heat Shock Protein 90 - 2168283 2168283
      Anti-Heat Shock Protein 90 - 2216912 2216912
      Anti-Heat Shock Protein 90 - 2283553 2283553
      Anti-Heat Shock Protein 90 - NRG1774101 NRG1774101
      Anti-Heat Shock Protein 90#945; - 2145104 2145104
      Anti-Heat Shock Protein 90#945; - 1996243 1996243
      Anti-Heat Shock Protein 90α - 2388980 2388980
      Anti-Heat Shock Protein 90α - 2895120 2895120
      Anti-Heat Shock Protein 90α - 3784497 3784497
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies