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PC319L Anti-Heparin Binding Epidermal Growth Factor (Ab-1) Goat pAb

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-HB-EGF

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PC319L
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HGtPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~19-23 kDa HB-EGF protein in vascular smooth muscle cells.
      Catalogue NumberPC319L
      Brand Family Calbiochem®
      SynonymsAnti-HB-EGF
      References
      ReferencesDavis-Fleischer, K.M. et al. 1998. Front. Biosci. 3, D288.
      Ouchi, N., et al. 1997. Biochem. J. 328, 923.
      Raab, G. and Klagsbrun M. 1997. Biochim. Biophys. Acta 1333, F179.
      Suzuki, M., et al. 1997. J. Biol. Chem. 272, 31730.
      Nakata, A., et al. 1996. Circulation 94, 2778.
      Miyagawa, J., et al. 1995. J. Clin. Invest. 95, 404.
      Product Information
      FormLyophilized
      FormulationLyophilized from sterile filtered solution in PBS, 5% trehalose, 100 µg BSA.
      Positive controlVascular smooth muscle cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Neutralization Studies
      Application NotesELISA (0.5-1 µg/ml, see comments)
      Immunoblotting (1-2 µg/ml)
      Neutralization Studies (see comments)
      Application CommentsThis antibody has been selected for its ability to neutralize the biological activity of human recombinant HB-EGF. It will not neutralize the biological activity of EGF, TGF-α or amphiregulin. The exact concentration of antibody required to neutralize human recombinant HB-EGF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of HB-EGF activity is ~3-6 µg/ml in the presence of 10 ng/ml of human recombinant HB-EGF using a Balb/3T3 cell line. For immunoblotting, the detection limit for human recombinant HB-EGF is ~0.5 ng/lane under non-reducing and reducing conditions. For ELISA, the detection limit for human recombinant HB-EGF is ~0.03 ng/well. This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified, recombinant, human HB-EGF
      ImmunogenHuman
      HostGoat
      IsotypeIgG
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 0.25 mg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC319L 0

      Documentation

      Anti-Heparin Binding Epidermal Growth Factor (Ab-1) Goat pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Heparin Binding Epidermal Growth Factor (Ab-1) Goat pAb Certificates of Analysis

      TitleLot Number
      PC319L

      References

      Reference overview
      Davis-Fleischer, K.M. et al. 1998. Front. Biosci. 3, D288.
      Ouchi, N., et al. 1997. Biochem. J. 328, 923.
      Raab, G. and Klagsbrun M. 1997. Biochim. Biophys. Acta 1333, F179.
      Suzuki, M., et al. 1997. J. Biol. Chem. 272, 31730.
      Nakata, A., et al. 1996. Circulation 94, 2778.
      Miyagawa, J., et al. 1995. J. Clin. Invest. 95, 404.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision09-October-2007 RFH
      SynonymsAnti-HB-EGF
      ApplicationELISA (0.5-1 µg/ml, see comments)
      Immunoblotting (1-2 µg/ml)
      Neutralization Studies (see comments)
      DescriptionPurified goat polyclonal antibody. Recognizes the ~19-23 kDa (apparent MW) heparin binding epidermal growth factor protein.
      BackgroundHeparin-binding epidermal growth factor-like growth factor (HB-EGF) is a ~22 kDa O-glycosylated protein that is a potent mitogen and chemoattractant for vascular smooth muscle cells, fibroblasts and epithelial cells but not endothelial cells. The natural protein has an apparent molecular mass of ~19-23 kDa and exists in multiple forms as a result of heterogeneous O-glycosylation and/or N-terminal truncation. HB-EGF is synthesized as a membrane-anchored precursor (proHB-EGF) that is proteolytically cleaved to release the soluble mature growth factor. The two forms are active as juxtacrine and paracrine/autocrine growth factors respectively. Recent studies show MMP-3 as one of the enzymes that cleaves HB-EGF implicating a role for MMP-3 in the regulation of HB-EGF from being a juxtacrine to a paracrine/autocrine factor. HB-EGF activates two EGF receptor subtypes, HER1/ErbB1 and HER4 and binds to heparan sulfate proteoglycan. The proHB-EGF is postulated as the high affinity receptor for diphtheria toxin (DT). Recent evidence suggests that the coexpression of proHB-EGF and CD9 on macrophages may strongly promote the development of atherosclerosis by a juxtacrine mechanism. Transcription of HB-EGF can be induced in vascular endothelial cells as well as aortic smooth muscle cells which may also play an important role in the pathogenesis of atherosclerosis.
      HostGoat
      Immunogen speciesHuman
      Immunogenpurified, recombinant, human HB-EGF
      IsotypeIgG
      Specieshuman
      Positive controlVascular smooth muscle cells
      FormLyophilized
      FormulationLyophilized from sterile filtered solution in PBS, 5% trehalose, 100 µg BSA.
      PreservativeNone
      CommentsThis antibody has been selected for its ability to neutralize the biological activity of human recombinant HB-EGF. It will not neutralize the biological activity of EGF, TGF-α or amphiregulin. The exact concentration of antibody required to neutralize human recombinant HB-EGF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of HB-EGF activity is ~3-6 µg/ml in the presence of 10 ng/ml of human recombinant HB-EGF using a Balb/3T3 cell line. For immunoblotting, the detection limit for human recombinant HB-EGF is ~0.5 ng/lane under non-reducing and reducing conditions. For ELISA, the detection limit for human recombinant HB-EGF is ~0.03 ng/well. This antibody exhibits no cross-reactivity with other cytokines when tested in ELISA. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 0.25 mg/ml. Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C. Avoid freeze/thaw cycles of solutions.
      Toxicity Harmful
      ReferencesDavis-Fleischer, K.M. et al. 1998. Front. Biosci. 3, D288.
      Ouchi, N., et al. 1997. Biochem. J. 328, 923.
      Raab, G. and Klagsbrun M. 1997. Biochim. Biophys. Acta 1333, F179.
      Suzuki, M., et al. 1997. J. Biol. Chem. 272, 31730.
      Nakata, A., et al. 1996. Circulation 94, 2778.
      Miyagawa, J., et al. 1995. J. Clin. Invest. 95, 404.