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PC544 Anti-Histone Deacetylase 1 (467-482) Rabbit pAb

Anti-Histone Deacetylase 1 (467-482), rabbit polyclonal, recognizes the ~62 kDa HDAC1 in HeLa, HepG2, DU145, PC3, and MCF-7 cells. It is validated for use in Western blotting and immunofluorescence.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-HDAC1

PC544
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      Ca, Ht, H, M, RRbPolyclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~62 kDa HDAC1 protein in HeLa, HepG2, DU145, PC3, and MCF-7 cells.
      Catalogue NumberPC544
      Brand Family Calbiochem®
      SynonymsAnti-HDAC1
      Application Data
      Detection of human histone deacetylase 1 by immunocytochemistry. Sample: HeLa cells. Primary antibody: Anti-Histone Deacetylase 1 (467-482) Rabbit pAb (Cat. No. PC544) (2 µg/ml). Detection: fluorescence.

      Detection of human Histone Deacetylease 1 by immunoblotting. Sample: Whole cell lysate from HeLa cell. Primary antibody: Anti-Histone Deacetylase 1 (467-482) Rabbit pAb (Cat. No. PC544) (0.5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesCress, W.D. and Seto, E. 2000. J Cell Physiol. 184, 1.
      Juan, L.J., et al. 2000. J Biol Chem. 275, 20436.
      Taunton, J., et al. 1996. Science 272, 408.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Positive controlHeLa, HepG2, Du145, PC-3, or MCF7 cells
      Preservative≤0.1% sodium azide
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Application NotesImmunoblotting (0.5 µg/ml)
      Immunocytochemistry (2 µg/ml)
      Immunofluorescence (1 µg/ml)
      Immunohistochemistry (0.2 µg/ml)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic peptide corresponding to amino acids 467-482 of human histone deacetylase 1
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Canine
      • Hamster
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      PC544 0

      Documentation

      Anti-Histone Deacetylase 1 (467-482) Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Histone Deacetylase 1 (467-482) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC544

      References

      Reference overview
      Cress, W.D. and Seto, E. 2000. J Cell Physiol. 184, 1.
      Juan, L.J., et al. 2000. J Biol Chem. 275, 20436.
      Taunton, J., et al. 1996. Science 272, 408.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision11-October-2007 RFH
      SynonymsAnti-HDAC1
      ApplicationImmunoblotting (0.5 µg/ml)
      Immunocytochemistry (2 µg/ml)
      Immunofluorescence (1 µg/ml)
      Immunohistochemistry (0.2 µg/ml)
      Application Data
      Detection of human histone deacetylase 1 by immunocytochemistry. Sample: HeLa cells. Primary antibody: Anti-Histone Deacetylase 1 (467-482) Rabbit pAb (Cat. No. PC544) (2 µg/ml). Detection: fluorescence.

      Detection of human Histone Deacetylease 1 by immunoblotting. Sample: Whole cell lysate from HeLa cell. Primary antibody: Anti-Histone Deacetylase 1 (467-482) Rabbit pAb (Cat. No. PC544) (0.5 µg/ml). Detection: chemiluminescence.
      DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~62 kDa HDAC1 protein.
      BackgroundThe regulation of eukaryotic gene expression is marked by post-translational modification of nucleosomal histones, a key event that converts regions of chromosomes into transcriptionally active or inactive chromatin. A single nucleosomal core particle contains an octamer of histone proteins H2A, H2B, H3 and H4. Acetylated histone proteins confer accessibility of the DNA template to the transcriptional machinery. There are at least eight cloned human histone deacetylases. Studies have pointed to the possible involvement of histone deacetylases in human cancer. Histone Deacetylase 1 has been shown to interact directly with transcription factors and to deacetylate histone proteins H3 and H4. Studies have also shown that mammalian histone deacetylase (HDAC)-1, -2, and -3 are all capable of down-regulating p53 function. p53, the most commonly mutated gene in cancer cells, directs cell cycle arrest or induces programmed cell death (apoptosis) in response to stress. HDACs are classified by their sequence homology to the yeast HDACs. Class I proteins are related to Rpd3 and members of class II resemble HdaIp.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic peptide corresponding to amino acids 467-482 of human histone deacetylase 1
      IsotypeIgG
      Speciescanine, hamster, human, mouse, rat
      Positive controlHeLa, HepG2, Du145, PC-3, or MCF7 cells
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesCress, W.D. and Seto, E. 2000. J Cell Physiol. 184, 1.
      Juan, L.J., et al. 2000. J Biol Chem. 275, 20436.
      Taunton, J., et al. 1996. Science 272, 408.